Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
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10.61186/ibj
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8888
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en
jalali
1398
4
1
gregorian
2019
7
1
23
4
online
1
fulltext
en
Hydroalcoholic Extract of Levisticum officinale Increases cGMP Signaling Pathway by Down-Regulating PDE5 Expression and Induction of Apoptosis in MCF-7 and MDA-MB-468 Breast Cancer Cell Lines
Cancer Biology
Cancer Biology
مقاله کامل
Full Length/Original Article
<strong>Background:</strong> This study aimed to investigate Levisticum officinale hydroalcoholic extract (<em>LOHE</em>) effect on both cGMP signaling pathway and phosphodiesterase 5 (<em>PDE5</em>) gene expression pattern and to examine the role of LOHE in apoptosis induction of MCF-7 and MDA-MB-468 cell lines. <strong>Methods</strong>: The half maximal inhibitory concentration (IC50) of <em>LOHE</em> was examined in both cell lines using the MTT assay. Using IC50 values of <em>LOHE</em> on both cells, the type of cell death was detected by flowcytometric analysis. The values of <em>PDE5</em> and cGMP were evaluated by real-time PCR and ELISA methods, respectively. <strong>Results</strong>: The IC50 values were measured as 150 μg/ml for MDA-MB-468 and 200 μg/ml for MCF-7. At 12 hour of treatment, a significant decrease in the <em>PDE5</em> expression and maximum increase in the amount of intracellular cGMP were observed (<em>p</em> < 0.05). However, these effects were more noticeable in MDA-MB-468 triple-negative cells. <strong>Conclusion:</strong> Our data suggest that <em>LOHE</em> extract could be a potential source for new strategies towards targeting both <em>PDE5</em> and cGMP signaling pathways.
Apoptosis, Breast cancer, Levisticum, PDE5
280
286
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-757&slc_lang=en&sid=1
Marzieh
Lotfian Sargazi
100319475328460054813
100319475328460054813
No
Cellular and Molecular Research Center Zahedan University of Medical Sciences, Zahedan, Iran
Ramin
Saravani
100319475328460054814
100319475328460054814
Yes
Cellular and Molecular Research Center Zahedan University of Medical Sciences, Zahedan, Iran
Ali
Shahraki
100319475328460054815
100319475328460054815
No
Department of Biology, Faculty of Science, University of Sistan and Baluchestan, Zahedan, Iran