en C595 به عنوان عامل کنتراست جدید تصاویر رزنانس مغناطیسی در تشخیص سلول‌های سرطان پستان (MCF-7) مقاله کامل Full Length/Original Article <span style="FONT-SIZE: 11pt"><strong><font face="times new roman,times,serif" size="2">Background: </font></strong></span><span style="FONT-SIZE: 11pt"><font face="times new roman,times,serif" size="2">The monoclonal antibody, C595, against breast cancer cell line was conjugated with cyclic anhydride gadolinium-diethylenetriaminepenta-acetic acid (Gd-cDTPAa) to produce Gd-DTPA-C595 and used as specific breast cancer cell line (MCF-7) contrast agents in magnetic resonance imaging (MRI). <span style="mso-spacerun: yes">&nbsp;</span><b>Methods: </b>After incubation of breast cancer cell line (MCF-7), with different contrast agents (Gd-DTPA-C595, Gd-DTPA,</font> <font face="times new roman,times,serif" size="2">Gd-H and GdCl₃) at 37</font></span><span style="FONT-FAMILY: Symbol FONT-SIZE: 11pt mso-ascii-font-family: 'Times New Roman' mso-hansi-font-family: 'Times New Roman' mso-char-type: symbol mso-symbol-font-family: Symbol"><span style="mso-char-type: symbol mso-symbol-font-family: Symbol"><font face="times new roman,times,serif" size="2">&deg;</font></span></span><span style="FONT-SIZE: 11pt"><font face="times new roman,times,serif" size="2">C for 12 h and twice washing, the T₁ relaxation times and the signal enhancement of washing solutions of different contrast agents are examined by nuclear magnetic resonance and results are compared. The percent of Gd that attached into the cell membrane of MCF-7 was</font> <font face="times new roman,times,serif" size="2">also measured by UV spectrophotometer. <b>Results: </b>The data indicate that the T₁ relaxation of washing solutions at 11.4 Tesla (500 MHz) in Gd-DTPA-C595 was greater than in Gd-DTPA solutions and was much greater than in control. These conjugates (Gd-DTPA-C595) show high specificity for breast cancer cell line (MCF-7). The gadolinium concentration in washing solutions measured using UV-spectrophotometer showed no gadolinium attached into the cell membrane in the GdCl₃ as control.<b><span style="mso-spacerun: yes">&nbsp; </span>Conclusion: </b>Good cell membrane uptakes</font> <font size="3"><font face="times new roman,times,serif" size="2">of Gd‑DTPA-C595 indicate selective delivery of this agent into the breast cancer cell membrane and have considerable potential in diagnostic MRI.</font><b><span style="mso-spacerun: yes">&nbsp;</span></b></font></span> Magnetic resonance imaging (MRI), Gadolinium, Monoclonal antibody, Breast cancer 209 213 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-191&slc_lang=en&sid=1 Daryoush Shahbazi-Gahrouei داریوش شهبازی گهرویی shahbazi@med.mui.ac.ir Yes Mariam Roufeh مریم روفه No Mohammad Bagher Tavakoli محمد باقر توکلی No