en Molecular Immunology & Vaccines Molecular Immunology & Vaccines مقاله کامل Full Length/Original Article <strong>Background:</strong> This study aimed to evaluate the reaction of organism of laboratory animals on deuterium-depleted drinking diet. To assess the cell energy metabolism, the effect of a liquid medium with different deuterium contents on isolated liver mitochondria of random bred rats and Wistar rats was studied. <strong>Methods:</strong> This experimental study on the effect of deuterium-depleted drinking water (DDW) on 16-week-old male Wistar rats lasted for four weeks. Energy metabolism of mitochondria was examined through the production of hydrogen peroxide using an Amplex&reg; Red Hydrogen Peroxide/Peroxidase Assay Kit. <strong>Results:</strong> Modification of isotope (deuterium-protium [D/H]) composition of rats&rsquo; blood and organ tissues with DDW (-705&permil;), introduced into rats&rsquo; diet within four weeks, led to the formation of isotope D/H gradient between blood plasma and organ tissues and affected isotope D/H exchange reactions on the adaptive processes. The isolated liver mitochondria from the random bred rats consumed DDW presented a maximum increase in H₂O₂production during the incubation in DDW medium. This increased level of H₂O₂ production was higher in the isolated liver mitochondria of the rats consuming natural deuterium content drinking water (-24&permil;).<strong> Conclusion:</strong> The obtained results indicate the possibility of nutritional correction of isotope D/H metabolism in blood by means of products with modified isotope composition, as well as the prospects of using isotope exchange reactions in case of imbalance in function of the body&#39;s defense systems in different generations of animals.&nbsp; Deuterium, Mitochondria, Rats, Tissues 129 141 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-750&slc_lang=en&sid=1 Basov Alexandr Alexandrovich 0000-0002-2262-4549 No Kuban State University, Krasnodar, Russian Federation, Russia Elkina Аnna Anatolyevna 0000-0002-9452-0132 Yes Kuban State University, Krasnodar, Russian Federation, Russia Samkov Andrei Alexandrovich 0000-0002-9347-8470 No Kuban State University, Krasnodar, Russian Federation, Russia Volchenko Nikita Nikolaevich 0000-0002-8012-6644 No Kuban State University, Krasnodar, Russian Federation, Russia Moiseev Arcadii Victorovich 0000-0001-7605-682 No Kuban State Agrarian University, Krasnodar, Russian Federation, Russia; 4The V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, Moscow, Russian Federation, Russia Fedulova Liliya Viacheslavovna 0000-0003-3573-930X No Kuban State University, Krasnodar, Russian Federation, Russia Baryshev Mikhail Gennadievich 0000-0002-2130-3516 No Kuban State University, Krasnodar, Russian Federation, Russia Dzhimak Stepan Sergeevich 0000-0003-2618-5376 No Kuban State University, Krasnodar, Russian Federation, Russia