en تمایز چند رده‌ای سلول‌های بنیادی مزانشیمی مشتق شده از سیاهرگ بند ناف نوزادان مقاله کامل Full Length/Original Article <span style="FONT-SIZE: 11pt"><strong><font face="times new roman,times,serif" size="2">Background</font></strong></span><span style="FONT-SIZE: 11pt"><font size="2"><font face="times new roman,times,serif">: Mesenchymal stem cells (MSC) are a very promising transplantable stem cell source for a variety of cell replacement therapies. As the main source of MSC is bone marrow (BM), most of studies have been done on BM-derived MSC (BM-MSC). Umbilical cord (UC)-derived MSC (UC-MSC) which are recently introduced, is one of the good alternative source for these cells. The objective of this study was to isolate and characterize UC-MSC from human UC veins and studying of their potential to differentiate into </font><font face="times new roman,times,serif">various cell types such as fat, bone, cartilage and neuronal cells.<span style="mso-spacerun: yes">&nbsp; </span><b>Methods:</b> In this way, a cell population was isolated from 20 UC veins using a solution of 0.1% collagenase type IV. After identification of isolated cells by immunocytochemical and flow cytometry methods, these cells were exposured with adipogenic, osteogenic, chondrogenic and neurogenic agents.</font></font> <font face="times new roman,times,serif" size="2">Resulted differentiated cells were detected using specific staining for each lineage and room temperature (RT)-PCR.<span style="mso-spacerun: yes">&nbsp; </span><b>Results:</b> Immunophenotypically, this cell population was positive for CD73, CD166, CD105 markers and </font></span><span style="FONT-FAMILY: Symbol FONT-SIZE: 11pt mso-ascii-font-family: 'Times New Roman' mso-hansi-font-family: 'Times New Roman' mso-char-type: symbol mso-symbol-font-family: Symbol"><span style="mso-char-type: symbol mso-symbol-font-family: Symbol"><font face="times new roman,times,serif" size="2">a</font></span></span><span style="FONT-SIZE: 11pt"><font face="times new roman,times,serif" size="2">-smooth muscle actin and negative for CD31, CD34, CD49d markers, von Willebrand factor and smooth muscle myosin. Exposure of these cells to adipogenic,</font> <font face="times new roman,times,serif" size="2">osteogenic, chondrogenic and neurogenic agents resulted in morphological changes followed by lineage-specific staining for each differentiated cell type. RT-PCR analysis showed that these differentiated cells express fat, bone, cartilage</font> <font face="times new roman,times,serif" size="3"><font size="2">and neuronal markers, respectively.<span style="mso-spacerun: yes">&nbsp; </span><b>Conclusion</b>: Altogether, these findings indicate that UC-MSC possess morphological, immunophenotypical</font> <font size="2">and cell differentiation capacities similar to BM-MSC and so they can be used more extensively</font></font><font face="times new roman,times,serif" size="2"> in cell based therapy protocols and <i>in vitro</i> differentiation study models.</font><span style="mso-spacerun: yes"><font size="3">&nbsp; </font></span></span> Mesenchymal stem cells (MSC), Cell differentiation, Umbilical cord (UC) 175 184 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-195&slc_lang=en&sid=1 Mehdi Kadivar مهدی کدیور No Shohreh Khatami شهره خاتمی sh-khatami@pasteur.ac.ir Yes Yousef Mortazavi یوسف مرتضوی No Mohammad Taghikhani محمد تقی خانی No Mohammad Ali Shokrgozar محمد علی شکرگزار No