other Related Fields Related Fields مقاله کوتاه Short Communication <strong>Background: </strong>Quantitation of <em>Helicobacter pylori</em> (Hp) in the gastric tissue is essential for assessment of vaccination/therapeutic regimens. <strong>Methods & Results:</strong> Here, the inhibitory effect of mouse gastric DNA (MgDNA) on amplification of Hp genomic DNA (HpDNA) was evaluated by spiking HpDNA with serial dilutions of MgDNA, which yielded concentrations of >10 ng/&micro;l and >0.63-10 ng/&micro;l of MgDNA, as inhibition and interference zones, respectively. Mice were then inoculated with varying doses of Hp and assessed at the inhibition-free concentration of 0.63 ng/&micro;l. The average Hp copy numbers per microgram of gastric tissue discriminated mice having received high vs. low dose inoculums (<em>p </em>< 0.001). Secondly, Hp copy numbers were quantitated in immunized mice, which demonstrated significantly lower numbers, in reference to controls (<em>p </em>= 0.006). <strong>Conclusion: </strong>Our method, bypassing the inhibition and/or interference imposed by MgDNA, was able to quantitate gastric tissue-colonizing Hp, segregating mice inoculated with low vs. high doses of Hp, as well as those immunized from controls. Amplification, Inhibition, Interference, Standard curve, Quantitative PCR 297 302 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-739&slc_lang=other&sid=1 Mehdi Alikhani No HPGC Research Group, Medical Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran Ebrahim Shafaie No HPGC Research Group, Medical Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran Esmat Mirabzadeh Ardakani 0000-0003-3636-0780 No Animal Sciences Lab, Department of Molecular Medicine, Pasteur Institute of Iran, Tehran, Iran Maryam Esmaeili No HPGC Research Group, Medical Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran Samaneh Saberi No HPGC Research Group, Medical Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran Mojgan Hatefi No HPGC Research Group, Medical Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran Marjan Mohammadi 0000-0002-5585-4519 Yes HPGC Research Group, Medical Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran