en Molecular Genetics & Genomics Molecular Genetics & Genomics مقاله کامل Full Length/Original Article <strong>Background: </strong>Differential expression profile of microRNAs (miRNAs) could be a diagnosis signature for monitoring gastric cancer (GC) progression. In this study, we focus on the comparison of expression levels of miR-21, miR-25, miR-93, miR-106b, and miR-375 during the sequential pattern of GC development, including normal gastric, gastric dysplasia, and GC sample. <strong>Methods: </strong>We used SYBR Green-based quantitative-PCR to quantify miRNAs expression. <strong>Results: </strong>Our analysis revealed the increased expression levels of miR-21 (<em>p</em> = 0.034), miR-25 (<em>p</em> = 0.0003), miR-93 (<em>p </em>= 0.0406), and miR-106b (<em>p </em>= 0.023) in GC samples. In addition, GC patients with positive lymph node metastasis showed the up-regulation of miR-25, miR-93, and miR-106b (<em>p</em> < 0.05). <strong>Conclusion: </strong>Our findings suggested that the expression of miR-21, miR-25, miR-93, and miR-106b altered in GC, and some of them may be further investigated as biomarkers for GC early detection and prognosis prediction.&nbsp; Biomarkers, microRNAs, Stomach cancer 367 373 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-735&slc_lang=en&sid=1 Pegah Larki 0000-0002-3947-8162 No Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Alireza Ahadi 0000-0000-0000-0000 No Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Ali Zare 0000-0000-0000-0000 No Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Shahriar Tarighi 0000-0000-0000-0000 No Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mahrokh Zaheri 0000-0000-0000-0000 No Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mojgan Souri 0000-0000-0000-0000 No Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mohammad Reza Zali 0000-0002-9027-4560 No Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran Hamid Ghaedi 0000-0002-5034-0586 No Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mir Davood Omrani 0000-0002-0673-9717 Yes Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran