en Related Fields Related Fields مقاله کامل Full Length/Original Article <strong>Background:</strong> Chinese hamster ovary (CHO) cell line is considered as the most common cell line in the biopharmaceutical industry because of its capability in performing efficient post-translational modifications and producing the recombinant proteins, which are similar to natural human proteins. The optimization of the upstream process via different feed strategies has a great impact on the target molecule expression and yield. <strong>Methods:</strong> To determine and understand the molecular events beneath the feed effects on the CHO cell, a label-free quantitative proteomic analysis was applied. The proteome changes followed by the addition of a designed amino acid feed to the monoclonal antibody producing CHO cell line culture medium were investigated. <strong>Results: </strong>The glutathione synthesis, the negative regulation of the programmed cell death, proteasomal catabolic process, and the endosomal transport pathway were up-regulated in the group fed with a designed amino acid feed compared to the control group. <strong>Conclusion:</strong> Our findings could be helpful to identify new targets for metabolic engineering. CHO cells, Glutathione, Monoclonal antibodies, Proteomics 385 393 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-723&slc_lang=en&sid=1 Fatemeh Torkashvand 0000-0003-3066-1144 Yes Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Fereidoun Mahboudi 0000-0002-3380-5257 No Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Manouchehr Vossoughi 0000-0000-0000-0000 No Department of Chemical and Petroleum Engineering, Biochemical and Bioenvironmental Research Center Sharif University of Technology, Tehran, Iran Elnaz Fatemi 0000-0000-0000-0000 No Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Seyed Masoud Moosavi Basri 0000-0000-0000-0000 No Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Behrouz Vaziri 0000-0002-0295-5922 No Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran