Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
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10.61186/ibj
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8888
-
en
jalali
1397
6
1
gregorian
2018
9
1
22
5
online
1
fulltext
en
Detection of Somatic Mutation in Exon 12 of DNA Polymerase β in Ovarian Cancer Tissue Samples
Cancer Biology
Cancer Biology
مقاله کوتاه
Short Communication
<strong>Background:</strong> DNA polymerase β (<em>pol β</em>) is a key enzyme of base excision repair pathway. It is a 1-kb gene consisting of 14 exons. Its catalytic part lies between exon 8 and exon 14. Exon 12 has a role in deoxyribonucleotide triphosphate selection for nucleotide transferase activity. <strong>Methods: </strong>Genomic DNA was isolated from ovarian carcinoma samples. Single strand conformation polymorphism method was used to detect mutation in genomic DNA. <strong>Results:</strong> Twenty-four patients of the 152 pair of tumor samples (15.8%) exhibited a point mutation (C→G) in position 725 in exon 12, which shifts proline to arginine (P242R). Statistical analysis showed a significant (<em>p</em> < 0.001) relationship between pol β mutation and the age of detection. <strong>Conclusion:</strong> This is a newly reported somatic mutation of pol β in ovarian carcinoma patients from India.
DNA polymerase β, Single nucleotide polymorphism, Ovarian cancer
355
359
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-722&slc_lang=en&sid=1
Kalyani
Khanra
100319475328460055104
100319475328460055104
No
Department of Biotechnology, Panskura Banamali College, Panskura RS, PIN 721152, Purba Medinipur, West Bengal, India
Indranil
Choudhuri
100319475328460055105
100319475328460055105
No
Department of Biotechnology, Panskura Banamali College, Panskura RS, PIN 721152, Purba Medinipur, West Bengal, India
Nandan
Bhattacharyya
100319475328460055106
100319475328460055106
Yes
Department of Biotechnology, Panskura Banamali College, Panskura RS, PIN 721152, Purba Medinipur, West Bengal, India