Iranian Biomedical Journal

en Detection of Somatic Mutation in Exon 12 of DNA Polymerase β in Ovarian Cancer Tissue Samples Cancer Biology Cancer Biology مقاله کوتاه Short Communication Background: DNA polymerase β (pol β) is a key enzyme of base excision repair pathway. It is a 1-kb gene consisting of 14 exons. Its catalytic part lies between exon 8 and exon 14. Exon 12 has a role in deoxyribonucleotide triphosphate selection for nucleotide transferase activity. Methods: Genomic DNA was isolated from ovarian carcinoma samples. Single strand conformation polymorphism method was used to detect mutation in genomic DNA. Results: Twenty-four patients of the 152 pair of tumor samples (15.8%) exhibited a point mutation (C→G) in position 725 in exon 12, which shifts proline to arginine (P242R). Statistical analysis showed a significant (p < 0.001) relationship between pol β mutation and the age of detection. Conclusion: This is a newly reported somatic mutation of pol β in ovarian carcinoma patients from India. DNA polymerase β, Single nucleotide polymorphism, Ovarian cancer 355 359 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-722&slc_lang=en&sid=1 Kalyani Khanra 0000-0000-0000-0000 No Department of Biotechnology, Panskura Banamali College, Panskura RS, PIN 721152, Purba Medinipur, West Bengal, India Indranil Choudhuri 0000-0000-0000-0000 No Department of Biotechnology, Panskura Banamali College, Panskura RS, PIN 721152, Purba Medinipur, West Bengal, India Nandan Bhattacharyya 0000-0000-0000-0000 Yes Department of Biotechnology, Panskura Banamali College, Panskura RS, PIN 721152, Purba Medinipur, West Bengal, India