en Cancer Biology Cancer Biology مقاله کامل Full Length/Original Article <strong>Background:</strong> This study aimed to evaluate the diagnostic value of outer dense fiber 4 (<em>ODF4</em>), melanoma-associated antigen A3 (<em>MAGEA3</em>), and <em>MAGEAB4</em> mRNAs in transitional cell carcinoma (TCC), using a small amount of cell reverse transcriptase-polymerase chain reaction (RT-PCR) on urinary exfoliated cells. <strong>Methods:</strong> We recruited a total of 105 suspected TCC patients and 54 sex- and age-matched non-TCC controls. The candidates&rsquo; genetic expression patterns were investigated with RT-PCR, while reverse transcription quantitative PCR was applied to quantify and compare each mRNA level between cases and control groups. <strong>Results:</strong> The sensitivity of <em>ODF4</em>, <em>MAGEA3</em>, and <em>MAGEAB4</em> RT-PCR was 54.8%, 63%, and 53.4%, whereas the specificity was 73.7%, 86%, and 94.7%, respectively. Combining <em>ODF4</em>, <em>MAGEA3</em>, and <em>MAGEAB4</em> RT-PCR offered a relatively higher sensitivity (83.6%). <strong>Conclusion:</strong> RT-PCR with <em>ODF4</em>, <em>MAGEA3</em>, and <em>MAGEAB4</em> on urinary exfoliated cells could provide clinicians with a promising method to improve TCC diagnosis, especially in the case of gross hematuria and catheterization. The method used here is non-invasive, simple and convenient, and unlike cytology, it does not rely directly on expert professional opinions. These features can be of particular importance to the management of TCC patients in whom regular and lifelong surveillance is required. Transitional cell carcinoma, Clinical markers, Cancer testis antigen, Human outer dense fiber of sperm tails 4 protein 160 170 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-688&slc_lang=en&sid=1 Mandana Afsharpad Yes Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran Mohammad Reza Nowroozi No Uro Oncology Research Center, Tehran University of Medical Sciences, Tehran, Iran Mohsen Ayati No Uro Oncology Research Center, Tehran University of Medical Sciences, Tehran, Iran Mojtaba Saffari No Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran Saeed Nemati No Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran Elham Mohebbi No Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran Leila Nekoohesh No Department of Medical Biotechnology, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran Kazem Zendehdel No Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran Mohammad Hossein Modarressi No Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran