en Related Fields Related Fields مقاله کوتاه Short Communication <strong>Background:</strong> The present study is the first comprehensive report of the Molluscum contagiosum virus (MCV) in Iran based on the molecular technique for differentiation and typing of the MCV1 and MCV2. <strong>Methods:</strong> Patients were diagnosed as having tumor-like genital warts less than 5 mm in diameter, and HIV seronegative samples were chosen for this cross-sectional study. TaqMan real-time PCR was used to identify MCV following clinical examination. Typing of the MCV-positive specimens was performed in the SNP A27451G region of <em>MC021L</em> gene. <strong>Results:</strong> Of 1470 samples, 114 (7.75%) samples were positive for the MCV. From MCV-positive samples, 71.05% sequences were found to be related to the MCV1 and 28.95% to the MCV2. <strong>Conclusion: </strong>This assay constitutes a reliable method for identification and typing of the MCV genomic variants that could be valuable for<br> reviewing the pathogenesis, molecular epidemiology, and the natural history of MCV-related situations. Molluscum contagiosum virus, Polymerase chain reaction, Single nucleotide polymorphism 129 133 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-687&slc_lang=en&sid=1 Sedigheh Taghinezhad-S Yes Research and Development (R&D) Department, Keyvan Virology Specialty Laboratory (KVSL), Tehran, Iran Amir Hossein Mohseni No Research and Development (R&D) Department, Keyvan Virology Specialty Laboratory (KVSL), Tehran, Iran Hossein Keyvani No Research and Development (R&D) Department, Keyvan Virology Specialty Laboratory (KVSL), Tehran, Iran Narges Ghobadi No Research and Development (R&D) Department, Keyvan Virology Specialty Laboratory (KVSL), Tehran, Iran