en Related Fields Related Fields مقاله کامل Full Length/Original Article <strong>Background: </strong>Acute myeloblastic leukemia (AML) is a clonal disorder due to bone marrow failure and uncontrolled proliferation of myeloid lineage. Acute promyelocytic leukemia (APL) is a subtype of AML. Heterocyclic compounds, such as indole, are considered as attractive candidates for cancer therapy, due to their abundance in nature and known biological activity. Sal-like protein (SALL4) is a zinc finger transcription factor involving in the multi-potency of stem cells, in the NB4 cell line. This study was aimed to evaluate the effects of basal indole and its new derivative, 2-(1-((2, 4-Aril)imino)-2,2,2-trifluoroethyl) phenyl-1H Indole-3- carbaldehyde (TFPHC), on the expression of <em>SALL4</em>. <strong>Methods:</strong> Cells were cultured and treated with different concentrations (75, 150, and 300 &micro;g/mL) of the new indole derivative and DMSO, as a vehicle control, for 24 and 48 hours. Cell proliferation was evaluated by using Trypan blue exclusion and MTT assays. The percentage of apoptotic cells was&nbsp;determined by flowcytometry analysis using the Annexin V/PI apoptosis detection kit; mRNA expression of <em>SALL4</em> was studied using absolute quantitative RT-PCR. <strong>Results:</strong> Our findings demonstrated the effects of new indole derivatives on <em>SALL4</em> mRNA expression. Expression of <em>SALL4</em> mRNA was significantly decreased at 75, 150, and 300 &micro;g/mL concentrations. <strong>Conclusion:</strong> SALL4 plays a role in the survival of APL cells. <em>SALL4</em> expression could be suppressed by the novel indole derivative. Additionally, <em>SALL4</em> gene suppression can serve as a target in APL therapy. SALL4 protein, Indoles, Leukemia, Acute promyelocytic 99 106 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-685&slc_lang=en&sid=1 Zahra Sheikhrezaei Yes Department of Hematology and Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Kerman University of Medical Sciences, Kerman, Iran Parisa Heydari No Department of Hematology and Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Kerman University of Medical Sciences, Kerman, Iran Alireza Farsinezhad No Department of Hematology and Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Kerman University of Medical Sciences, Kerman, Iran Ahmad Fatemi No Department of Hematology and Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Kerman University of Medical Sciences, Kerman, Iran Soudeh Khanamani Falahati-Pour No Pistachio Safety Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran Shokoofeh Darakhshan No Department of Pediatrics, Rafsanjan University of Medical Sciences, Rafsanjan, Iran Mojgan Noroozi Karimabad No Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran Ali Darekordi No Department of Chemistry, Faculty of Science, Vali-E-Asr University of Rafsanjan, Rafsanjan, Iran Hossein Khorramdelazad No Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran Gholamhossein Hassanshahi No Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran