Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
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10.61186/ibj
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8888
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en
jalali
1396
10
1
gregorian
2018
1
1
22
1
online
1
fulltext
en
Low Level of Autophagy-Related Gene 10 (ATG10) Expression in the 6-Hydroxydopamine Rat Model of Parkinson's Disease
Related Fields
Related Fields
مقاله کامل
Full Length/Original Article
<p><strong>Background:</strong> Autophagy is a mechanism disassembling the damaged organelles from the cell. This study attempted to examine the expression of several autophagy-related genes in Parkinson’s disease (PD) rat model. <strong>Methods:</strong> The male Wistar rats were divided into three groups as control, sham, and lesion. In the latter group, the PD rat model was induced by the injection of 6-hydroxydopamine in the striatum. The behavioral test was conducted one (baseline) and four weeks after the surgery through apomorphine hydrochloride. Then the RT-PCR technique was employed to evaluate the expressions of <em>p62/SQSTM</em>, autophagy-related genes<em> (ATG)5</em>, <em>ATG12</em>, <em>ATG16L1</em>, <em>ATG10</em>, as well as <em>GAPDH</em> and <em>LC3</em>. <strong>Results:</strong> By injecting apomorphine, the striatal lesion group showed a significant contralateral rotation at fourth week as compared to the baseline. The examination of <em>p62</em>, <em>ATG5</em>, <em>ATG12</em>, <em>ATG16L1</em>, and <em>LC3</em> expressions using RT-PCR revealed that <em>p62</em>, <em>ATG5</em>, <em>ATG12</em>, <em>LC3</em>, and<em> ATG16L1</em> were expressed in the substantia nigra of PD rat model, while <em>ATG10</em> was not expressed. <strong>Conclusion:</strong> ATG10 expression is necessary for the initiation of autophagy. Thus, these results show that autophagy deregulation occurs in the initiation stages of the process in the rat model of PD.</p>
Parkinson’s disease, autophagy, 6-hydroxydopamine (6-OHDA),ATG16L, ATG10
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21
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-678&slc_lang=en&sid=1
Marzieh
Shams Nooraei
100319475328460055330
100319475328460055330
No
Cellular and Molecular Research Center, Qazvin University of Medical Science, Qazvin, Iran
Ali
Noori-Zadeh
100319475328460055331
100319475328460055331
No
Department of Clinical Biochemistry, Faculty of Medicine, Ilam University of Medical Sciences, Ilam,Iran
Shahram
Darabi
100319475328460055332
100319475328460055332
Yes
Cellular and Molecular Research Center, Qazvin University of Medical Science, Qazvin, Iran
Farzad
Rajaei
100319475328460055333
100319475328460055333
No
Cellular and Molecular Research Center, Qazvin University of Medical Science, Qazvin, Iran
Zohreh
Golmohammadi
100319475328460055334
100319475328460055334
No
Cellular and Molecular Research Center, Qazvin University of Medical Science, Qazvin, Iran
Hojjat Allah
Abbaszadeh
100319475328460055335
100319475328460055335
No
Hearing Disorder Research center, Shahid Beheshti University of Medical Science,Tehran, Iran