en Related Fields Related Fields مقاله کامل Full Length/Original Article <p><strong>Background:</strong> Autophagy is a mechanism disassembling the damaged organelles from the cell. This study attempted to examine the expression of several autophagy-related genes in Parkinson&rsquo;s disease (PD) rat model. <strong>Methods:</strong> The male Wistar rats were divided into three groups as control, sham, and lesion. In the latter group, the PD rat model was induced by the injection of 6-hydroxydopamine in the striatum. The behavioral test was conducted one (baseline) and four weeks after the surgery through apomorphine hydrochloride. Then the RT-PCR technique was employed to evaluate the expressions of <em>p62/SQSTM</em>, autophagy-related genes<em> (ATG)5</em>, <em>ATG12</em>, <em>ATG16L1</em>, <em>ATG10</em>, as well as <em>GAPDH</em> and <em>LC3</em>. <strong>Results:</strong> By injecting apomorphine, the striatal lesion group showed a significant contralateral rotation at fourth week as compared to the baseline. The examination of <em>p62</em>, <em>ATG5</em>, <em>ATG12</em>, <em>ATG16L1</em>, and <em>LC3</em> expressions using RT-PCR revealed that <em>p62</em>, <em>ATG5</em>, <em>ATG12</em>, <em>LC3</em>, and<em> ATG16L1</em> were expressed in the substantia nigra of PD rat model, while <em>ATG10</em> was not expressed. <strong>Conclusion:</strong> ATG10 expression is necessary for the initiation of autophagy. Thus, these results show that autophagy deregulation occurs in the initiation stages of the process in the rat model of PD.</p> Parkinson’s disease, autophagy, 6-hydroxydopamine (6-OHDA),ATG16L, ATG10 15 21 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-678&slc_lang=en&sid=1 Marzieh Shams Nooraei No Cellular and Molecular Research Center, Qazvin University of Medical Science, Qazvin, Iran Ali Noori-Zadeh No Department of Clinical Biochemistry, Faculty of Medicine, Ilam University of Medical Sciences, Ilam,Iran Shahram Darabi Yes Cellular and Molecular Research Center, Qazvin University of Medical Science, Qazvin, Iran Farzad Rajaei No Cellular and Molecular Research Center, Qazvin University of Medical Science, Qazvin, Iran Zohreh Golmohammadi No Cellular and Molecular Research Center, Qazvin University of Medical Science, Qazvin, Iran Hojjat Allah Abbaszadeh No Hearing Disorder Research center, Shahid Beheshti University of Medical Science,Tehran, Iran