Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
-
10.61882/ibj
-
8888
-
en
jalali
1396
10
1
gregorian
2018
1
1
22
1
online
1
fulltext
fa
Analysis of NSP4 Gene and Its Association with Genotyping
of Rotavirus Group A in Stool Samples
Molecular Microbiology
Molecular Microbiology
مقاله کامل
Full Length/Original Article
<p><strong>Background</strong>: Non-structural protein 4 (<em>NSP4</em>) is a critical protein for rotavirus (RV) replication and assembly. This protein has multiple domains and motifs that predispose its function and activity. <em>NSP4</em> has a sequence divergence in human and animal RVs. Recently, 14 genotypes (E1-E14) of <em>NSP4</em> have been identified, and E1 and E2 have been shown to be the most common genotypes in human. <strong>Methods:</strong> The gene and protein sequence of <em>NSP4</em> in RV-positive samples were inspected with the aim of <em>NSP4</em> genotyping and variation analysis in viroporin and other domains. P and G typings of RV samples were carried out by WHO primers using a semi-multiplex PCR method. Non-typeable RV samples were amplified by conserved primers and sequenced. <strong>Results: </strong>In viroporin and enterotoxin, conserved sequence was detected, and amino acids substitution with the same biochemical properties was found. <strong>Conclusion</strong>: Association of <em>NSP4</em> genotype with P or G genotyping G1/G9 correlates with E1 genogroups. In electrophoretyping of RV, E2 genotype had a short pattern when compared to E1.</p>
NSP4, Rotavirus, Genotyping
42
49
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-676&slc_lang=fa&sid=1
Ali
Teimoori