en Pharmaceutical Biotechnology Pharmaceutical Biotechnology مقاله کوتاه Short Communication <p><strong>Background:</strong> Design of experiments is a rapid and cost-effective approach for optimization of recombinant protein production process. In our previous study, we generated a potent dual-acting fusion protein, anti-CD22 scFv-apoptin, to target B-cell malignant cell lines. In the present investigation, we report the effect of different variables on the expression levels of this fusion protein. <strong>Methods:</strong> Four variables (cell optical density at induction, IPTG concentration, induction temperature, and induction time) were tested using experimental design. <strong>Results:</strong> Our findings demonstrated that among the examined variables, only the induction time had a significant positive effect on the protein expression yield. <strong>Conclusion:</strong> Experimental design was successfully applied in this study. The optimized condition obtained in the current study can be applied in future commercial production of this novel fusion protein.</p> Recombinant protein, Single-chain antibodies, Fusion proteins, E. coli 66 69 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-675&slc_lang=en&sid=1 Solmaz Agha Amiri Yes Department of Pharmaceutical Biotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran Najmeh Zarei No Department of Medical Biotechnolgy, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Somayeh Enayati No Department of Medical Biotechnolgy, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Mohammad Azizi No Department of Medical Biotechnolgy, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Vahid Khalaj No Department of Medical Biotechnolgy, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Soraya Shahhosseini No Department of Pharmaceutical Chemistry, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran