Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
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10.61882/ibj
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8888
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en
jalali
1396
8
1
gregorian
2017
11
1
21
6
online
1
fulltext
en
In vitro Study of SPIONs-C595 as Molecular Imaging Probe for Specific Breast Cancer (MCF-7) Cells Detection
Medical Biotechnology
Medical Biotechnology
مقاله کامل
Full Length/Original Article
<p><strong>Background:</strong> Magnetic resonance imaging (MRI) plays an essential role in molecular imaging by delivering the contrast agent into targeted cancer cells. The aim of this study was to evaluate the C595 monoclonal antibody-conjugated superparamagnetic iron oxide nanoparticles (SPIONs-C595) for the detection of breast cancer cell (MCF-7). <strong>Methods:</strong> The conjugation of monoclonal antibody and nanoparticles was confirmed using X-ray diffraction, transmission electron microscopy, and photon correlation spectroscopy. The selectivity of the nanoprobe for breast cancer cells (MCF-7) was obtained by Prussian blue, atomic emission spectroscopy, and MRI relaxometry. <strong>Results:</strong> The <em>in vitro </em>MRI showed that T<sub>2</sub> relaxation time will be reduced 76% when using T<sub>2</sub>-weighed magnetic resonance images compared to the control group (untreated cells) at the dose of 200 μg Fe/ml, as the optimum dose. In addition, the results showed the high uptake of nanoprobe into MCF-7 cancer cells. <strong>Conclusion:</strong> The SPIONs-C595 nanoprobe has potential for the detection of specific breast cancer. </p>
Nanoparticles, Molecular Imaging, MCF-7 cells, Monoclonal antibody
360
368
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-668&slc_lang=en&sid=1
Pegah
Moradi Khaniabadi