en Related Fields Related Fields مقاله کامل Full Length/Original Article <p><strong>Background</strong>: With considering the importance of natural products for their remedial and therapeutic value, this research was aimed to analyze the chemical compositions and antimicrobial activity of four propolis samples from different areas of Iran (Chenaran, Taleghan, Morad Beyg, and Kalaleh) with various climates and flora. <strong>Methods</strong>: Ethanolic (70% EtOH) and dichlromethane (DCM) extracts of Iranian propolis were analyzed by gas chromatography-mass spectrometry (GC-MS) methods, and antimicrobial activity was evaluated against <em>Candida albicans, Escherichia coli, </em>and <em>Staphylococcus aureus </em>using disk diffusion antimicrobial method. <strong>Results:</strong> The results of GC-MS analysis showed the presence of fatty acids, flavonoids, terpenes, aromatic-aliphatic acids, and their related esters. The total flavonoids in DCM extract of Chenaran, Taleghan, Morad Beyg, and Kalaleh propolis were pinocembrin and pinostrobin chalcone. The common phenolic and terpene compounds detected in all four tested EtOH extracts were <em>P</em>-cumaric acid and dimethyl -1,3,5,6-tetramethyl-[1,3-(13C2)] bicycloce [5.5.0] dodeca-1,3,5,6,8,10-hexaene-9,10-dicarboxylate, respectively. The highest inhibitory diameter zone of the Iranian propolis against <em>C. albicans</em>, <em>E. coli</em>, and<em> S. aureus </em>was for DCM extract of Kalaleh propolis (13.33 mm), Morad Beyg propolis (12 mm), and Kalaleh (11.67 mm), respectively. <strong>Conclusion</strong>: Iranian propolis showed antimicrobial activities against <em>C. albicans</em>, <em>E. coli</em>, and <em>S. aurous</em>, perhaps due to the presence of flavonoids, phenolic acids, and terpenes as active components that can be used alone or in combination with the selected antibiotics to<br> synergize antibiotic effect, as well as to prevent microbial resistance to available antimicrobial drugs.</p> Propolis, Gas chromatography-mass spectrometry, Flavonoids, Iran 50 65 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-667&slc_lang=en&sid=1 Houshang Afrouzan Yes Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), Pasteur Institute of Iran, Tehran, Iran Azar Tahghighi No Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), Pasteur Institute of Iran, Tehran, Iran Sedigheh Zakeri No Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), Pasteur Institute of Iran, Tehran, Iran Ali Es-haghi No Department of Physico Chemistry, Razi Vaccine and Serum Research Institute Karaj, Iran