Iranian Biomedical Journal

en Expression Study and Clinical Correlations of MYC and CCAT2 in Breast Cancer Patients Cancer Biology Cancer Biology مقاله کامل Full Length/Original Article Background: Colon cancer-associated transcript 2 (CCAT2) is a newly recognized lncRNA transcribed from the 8q24 genomic region. It functions as an oncogene in various types of cancers including breast cancer, in which it affects Wnt/β-catenin pathway. Previous studies have shown a putative interaction between this lncRNA and MYC proto-oncogene. Methods: In the current study, we evaluated the expression of CCAT2 in breast cancer tissues with regards to the expression of its target MYC. In addition, we assessed the relationship between CCAT2 and MYC expression levels in tumor tissues and the clinical prognostic characteristics of breast cancer patients. Results: MYC expression levels were significantly up-regulated in tumor tissues compared with adjacent non-cancerous tissues (ANCTs), while such analysis showed no statistically significant difference between these two tissue types in CCAT2 expression. Starkly increased CCAT2 gene expression levels were found in 12/48 (25%) of cancer tissue samples compared with their corresponding ANCTs. Furthermore, significant inverse correlations were found between CCAT2 expression and stage, as well as lymph node involvement. Besides, a significant inverse correlation was found between the relative MYC expression in tumor tissues compared with their corresponding ANCTs and disease stage. Conclusions: These results highlight the significance of MYC and CCAT2 expressions in the early stages of breast cancer development and suggest a potentially significant role for CCAT2 in a subset of breast cancer patients, which could applied as a potential therapeutic target in these patients. Long non-coding RNA, Breast cancer, c-MYC 303 311 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-659&slc_lang=en&sid=1 Shaghayegh Sarrafzadeh No Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran Lobat Geranpayeh No Department of Surgery, Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran Behnoosh Tasharrofi No Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mohammad Soudyab No Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran Elahe Nikpayam No Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mostafa Iranpour No Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran Reza Mirfakhraie No Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran Jalal Gharesouran No Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran Somayyeh Ghafouri-Fard No Department of Community and Preventive Medicine, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Soudeh Ghafouri-Fard Yes Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran