en Molecular Microbiology Molecular Microbiology مقاله کوتاه Short Communication <p><strong>Background:</strong> Detection and quantification of human Papillomavirus (HPV) genome in oral carcinoma play an important role in diagnosis, as well as implications for progression of disease. <strong>Methods:</strong> We evaluated tissues from 50 esopharyngeal cancers collected from different regions of Iran for HPV E6 using the two type-specific primers sets. <em>E6</em> gene of HPV genotypes was amplified by specific primers. The sensitivity of PCR assay was analyzed and determined using HPV-DNA-containing plasmids. Real-time PCR was utilized to determine the prevalence and HPV viral load in patients with oral cavity squamous cell carcinoma. <strong>Results:</strong> Eighteen (36%) specimens were positive for HPV. Among the 18 positive specimens, 10 showed HPV-18 (55.55%), and 8 specimens were positive for HPV-11 (44.44%). Of the 18 infected specimens, 6 (33.32%) and 12 (66.65%) were identified as high-titer and low-titer viral load, respectively.<strong> Conclusions:</strong> The PCR-based assay, developed in the current study, could be used for HPV detection, quantification, and genotyping in epidemiological and clinical studies.</p> Real-time PCR, Genotyping, Iran 411 416 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-2438-2&slc_lang=en&sid=1 Hadi Razavi Nikoo No Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, Iran Abdollah Ardebili No Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, Iran Mehrdad Ravanshad Yes Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Fatemeh Rezaei No Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Ali Teimoori No Health Research Institute, Infectious and Tropical Diseases Reseach Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Sayyad Khanizadeh No Hepatitis Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran Mohammad Hassan Pouriayevali No Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran Mehdi Ajorloo No Hepatitis Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran