Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
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10.61186/ibj
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8888
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en
jalali
1396
2
1
gregorian
2017
5
1
21
3
online
1
fulltext
fa
An Increased Genotoxic Risk in Lymphocytes from Phototherapy-Treated Hyperbilirubinemic Neonates
Molecular Genetics & Genomics
Molecular Genetics & Genomics
مقاله کامل
Full Length/Original Article
<p><strong>Background: </strong>Phototherapy is believed to be a safe method for the management of hyperbilirubinemia. However, there are some controversial issues regarding the genotoxic effects of phototherapy on DNA. The aim of this study was to investigate morphologically both phototherapy-induced DNA double-strand breaks (DSBs) and apoptosis in lymphocytes derived from jaundiced and non-jaundiced neonates.<strong> Methods: </strong>Newborns were divided into three groups, including phototherapy-treated (PT, n=30) jaundiced newborns with total serum bilirubin (TSB) levels >15 mg/dl, non-treated jaundiced newborns (C<strong><sup>+</sup></strong>, n=27), as positive, as well as healthy negative (C<sup>-</sup>, n=30) controls with TSB levels ranging from 10 and 15 mg/dl and less than 5 mg/dl, respectively. Lymphocytes were isolated from whole blood samples by Ficoll-isopaque density gradient centrifugation and then assessed for DNA damage and apoptosis before and 24 hours after incubation at 37°C in 5% CO<sub>2 </sub>using the neutral comet assay. <strong>Results:</strong> DSB levels were significantly much higher in the PT group compared to the controls before incubation but decreased remarkably after the incubation period. As expected, no statistical differences were found between the two control groups before and after incubations. The frequency of apoptotic cells showed no significant differences among all the three groups before incubation; however, it was significantly increased in the PT group after incubation. <strong>Conclusion: </strong>It seems that phototherapy in jaundiced infants is able not only to induce apoptosis in newborn lymphocytes but also to affect indirectly DNA integrity.</p>
Phototherapy, Hyperbilirubinemic, Neonates, Apoptosis
182
189
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-637&slc_lang=fa&sid=1
Seyed Alireza
Mesbah-Namin
100319475328460055557
100319475328460055557
No
Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Maryam
Shahidi
shahidi_maryam@yahoo.com
100319475328460055558
100319475328460055558
Yes
Department of Biochemistry and Biophysics, Faculty of Medicine, Mazandaran University of Medical Sciences, Mazandaran, Sari, Iran
Maryam
Nakhshab
100319475328460055559
100319475328460055559
No
Department of Pediatrics, Faculty of Medicine, Mazandaran University of Medical Sciences, Mazandaran, Sari, Iran