en Production of Non-Ribosomal Peptide Synthetase (NRPS)- Dependent Siderophore by Aeromonas Isolates Molecular Microbiology Molecular Microbiology مقاله کامل Full Length/Original Article <p style="direction: ltr; "><strong>Background</strong><em>: Aeromonas</em> species are Gram-negative ubiquitous bacteria, facultative anaerobic rods that infect both invertebrates and vertebrates. Various fish species develop hemorrhagic disease and furunculosis due to <em>Aeromonas </em>spp. <em>Aeromonas</em> strains generate certain active compounds such as siderophores, which are the final products of non-ribosomal peptide synthetase (NRPS) activity. The present study attempted to investigate the prevalence of <em>Aeromonas</em> isolates in marketed fish sources. We also examined the siderophore production ability of these isolates. <strong>Methods</strong>: Among the molecular tools, 16S rRNA analysis was used to identify <em>Aeromonas</em> species and their epidemiological distributions. The hemolytic activity of the strains and biochemical assays were used to confirm the identity of the isolates. We also determined the chemical nature of siderophores in these strains. <strong>Results:</strong> A total of seven <em>Aeromonas</em> isolates obtained from fish were included to determine the siderophore production. Of 7 isolates, 4 produced siderophore, and their chemical nature was also determined. The siderophore produced by <em>Aeromonas</em> was invariably found to be of hydroxamate. Four <em>Aeromonas</em> isolates were selected for PCR identification of NRPS-encoding gene. The conserved sequence was present in all four selected isolates. Furthermore, siderophores were qualitatively tested for their antibacterial activity against pathogenic bacteria and a significant level of inhibitory activity was observed in siderophores from the four isolates. <strong>Conclusion:</strong> Our results showed the ability of the isolated strains in production of siderophores with a high level of activity against <em>Salmonella paratyphi</em>. These siderophores could find applications in biomedical industries.</p> Aeromonas, Siderophores, Hydroxamate 235 240 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-610&slc_lang=en&sid=1 Ramasamy Amsaveni No Muthusamy Sureshkumar No Arthanari Aravinth No Joseph Reshma Mary No Govindasami Vivekanandhan biovivek2015@gmail.com Yes