en Cytoprotective Effect of Hydroalcoholic Extract of Pinus eldarica Bark against H2O2-Induced Oxidative Stress in Human Endothelial Cells Related Fields Related Fields مقاله کامل Full Length/Original Article <p style="direction: ltr; "><strong>Background: </strong><em>Pinus eldarica</em> is a widely growing pine in Iran consisting of biologically active constituents with antioxidant properties. This study investigates the effect of hydroalcoholic extract of <em>P. eldarica </em>bark against oxidative damage induced by hydrogen peroxide (H₂O₂) in human umbilical vein endothelial cells (HUVECs). <strong>Methods:</strong> The total phenolic content of <em>p. eldarica</em> extract was determined using Folin-Ciocalteu method. The cytotoxicity of <em>P. eldarica </em>extract (25-1000 &micro;g/ml) on HUVECs was assessed using 3-(4,5- Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) method. Cytoprotective effect of <em>P. eldarica </em>extract (25-500 &micro;g/ml) on H₂O₂-induced oxidative stress was also evaluated by MTT assay. The intra- and extra-cellular hydroperoxides concentration and ferric reducing antioxidant power (FRAP) were measured in pretreated cells. <strong>Results: </strong>The total phenolic content of <em>P. eldarica </em>extract was estimated as 37.04&plusmn;1.8% gallic acid equivalent. <em>P. eldarica</em> extract (25-1000 &micro;g/ml) had no cytotoxic effect on HUVECs viability. The pretreatment of HUVECs with <em>P. eldarica </em>extract at the concentrations of 50-500 &micro;g/ml significantly reduced the cytotoxicity of H₂O₂. <em>P. eldarica</em> extract decreased hydroperoxides concentration and increased FRAP value in intra-cellular fluid at the concentration range of 100-500 &micro;g/ml and in extra-cellular fluid at the concentration range of 25-500 &micro;g/ml. <strong>Conclusions:</strong> This study revealed the antioxidant and cytoprotective effects of <em>P. eldarica </em>extract against H₂O₂-induced oxidative stress in HUVECs. &nbsp;Concerning the high content of phenolic compounds in<em> P. eldarica</em>, more research is needed to evaluate its clinical value in endothelial dysfunction and in other oxidative conditions.&nbsp;</p> Pinus eldarica, human umbilical vein endothelial cells, Oxidative stress, Antioxidants 161 167 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-618&slc_lang=en&sid=1 Fatemeh Babaee No Leila Safaeian leila_safaeian@pharm.mui.ac.ir Yes Behzad Zolfaghari No Shaghayegh Haghjoo Javanmard No