Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
-
10.61186/ibj
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8888
-
en
jalali
1395
1
1
gregorian
2016
4
1
20
2
online
1
fulltext
en
Utilization of Site-Specific Recombination in Biopharmaceutical Production
Utilization of Site-Specific Recombination in Biopharmaceutical Production
Related Fields
Related Fields
مقاله مروری
Review Article
<p style="direction: ltr ">Mammalian expression systems, due to their capacity in post-translational modification, are preferred systems for biopharmaceutical protein production. Several recombinant protein systems have been introduced to the market, most of which are under clinical development. In spite of significant improvements such as cell line engineering, introducing novel expression methods, gene silencing and process development, expression level is unpredictable and unstable because of the random location of integration in the genome. Site-specific recombination techniques are capable of producing stable and high producer clonal cells therefore, they are gaining more importance in the biopharmaceutical production. Site-specific recombination methods increase the recombinant protein production by specifically inserting a vector at a locus with specific expression trait. The present review focused on the latest developments in site-specific recombination techniques, their specific features and comparisons.</p>
Site-specific recombinase, Gene targeting, CHO cell
68
76
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-588&slc_lang=en&sid=1
Maryam
Ahmadi
100319475328460055782
100319475328460055782
No
Narges
Damavandi
100319475328460055783
100319475328460055783
No
Mohammad Reza
Akbari Eidgahi
100319475328460055784
100319475328460055784
No
Fatemeh
Davami
f_davami@pasteur.ac.ir
100319475328460055785
100319475328460055785
Yes