en A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (cop-GFP) in Baculovirus Expression System Molecular Genetics & Genomics Molecular Genetics & Genomics مقاله کوتاه Short Communication <p style="direction: ltr; "><strong>Background:</strong> Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells. <strong>Methods:</strong> The aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluorescent protein (copGFP). In this light, the resultant vector was constructed and used for transfection of <em>Spodoptera frugiperda</em> cells. <strong>Results:</strong> Expression of the copGFP protein in insect cells was confirmed by fluorescent microscopy and Western-blot analysis. <strong>Conclusion:</strong> The application of copGFP control bacmid can be considered as an appropriate control for insect cell transfection.</p> <p style="direction: ltr; "></p> Baculovirus, Sf9, Transfection 182 186 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-582&slc_lang=en&sid=1 Narjes Shokrollahi No Delavar Shahbazzadeh No Kamran Pooshang-Bagheri No Mahdi Habibi-Anbouhi No Ali Jahanian-Najafabadi No Mahdi Behdani Behdani73042@yahoo.com Yes