en مقایسه تکوین جنین حاصل از روش روتین تزریق درون سیتو پلاسمی اسپرم با روش تزریق اسپرم با تکنیک پیزو بعد از تیمار اسپرم موش با کلسیم یونوفور Related Fields Related Fields مقاله کامل Full Length/Original Article The purpose of this study was to evaluate the efficiency of intracytoplasmic sperm injection (ICSI) and Piezo-assisted sperm injection after pretreatment with calcium ionophore (CaI) on the mouse embryo development. Methods: In this study, the conventional ICSI and Piezo-ICSI procedures were used. The efficacy of the methods was examined after mouse matured oocytes were fertilized with or without CaI-treated sperms. Results: Piezo-ICSI demonstrated significantly more favorable results, with a fertilization rate of 64% (conventional ICSI: 42%, P<0.001) and a cleavage rate of 73% (conventional ICSI: 58%, P<0.05). When the Piezo-ICSI procedure was performed with CaI-pretreated sperms, the cleavage rate significantly increased (92% vs. 73%, P<0.05). However, the fertilization rate did not change significantly (64% vs. 56%). Conclusion: The Piezo-ICSI accompanies with CaI-treated sperms is more efficient than the conventional ICSI method for fertilizing and thus obtaining more mouse embryos. Intracytoplasmic sperm injection (ICSI), Piezo, Sperm activation, Embryo development 245 250 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-108&slc_lang=en&sid=1 Abdolhossein Shahverdi عبدالحسین شاهوردی No Mansoureh Movahedin منصوره موحدین mansoure@modares.ac.ir Yes Mojtaba Rezazadeh Valojerdi مجتبی رضازاده ولوجردی No Saeid Kazemi Ashtiani سعید کاظمی آشتیانی No