en Related Fields Related Fields مقاله کامل Full Length/Original Article <p>Background: &beta-thalassemia is the most common monogenic disorder in human. The (CT) polymorphism at -158 upstream region of the &gammaG-globin gene and pharmacological factors such as hydroxyurea have been reported to influence &gamma-globin gene expression and the severity of clinical symptoms of &beta-thalassemia. Methods: In the present study, 51 &beta-thalassemia intermediate patients were studied. Xmn1&gammaG polymorphism genotype was determined using Tetra-Primer ARMS-PCR technique. Hemoglobin (Hb) and fetal hemoglobin (HbF) levels were determined by gel electrophoresis. Results: Of 51 patients, 35 (68.6%) patients were heterozygous (CT) and 16 (31.4%) patients were homozygous (CC). Of 30 patients under treatment by hydroxyurea, 20 (66.7%) patients were heterozygous (CT) and 10 (33.3%) patients were homozygous (CC). Our results demonstrated that in the heterozygous (CT) genotype, the Hb (9.58 &plusmn; 1.25 gm/dl) and HbF (89.30 &plusmn; 21.87) levels were significantly higher in comparison with homozygous (CC) genotype (7.94 &plusmn; 1.34 gm/dl and 70.32 &plusmn; 40.56, respectively). Furthermore, we observed that after drug usage, the Hb and HbF levels in patients with heterozygous (CT) genotype (0.7 &plusmn; 1.26 gm/dl and 5.95&plusmn;14.8, respectively) raised more in comparison with homozygous (CC) genotype (0.26 &plusmn; 1.43 gm/dl and 0.8&plusmn;1.31, respectively). Conclusion: Hb and HbF levels in the patients carrying T allele are increased significantly, and they also response to hydroxyurea treatment.</p> Fetal hemoglobin (HbF), Hydroxyurea, Intermediate β-thalassemia 177 182 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-548&slc_lang=en&sid=1 Majid Motovali-Bashi mbashi@sci.ui.ac.ir Yes Tayyebeh Ghasemi No