en مقایسه میزان رونویسی ژن های میتوکندریایی TFAM, NRF1 و MT-CO1 در مراحل مختلف بلوغ تخمک های انسان Related Fields Related Fields مقاله کامل Full Length/Original Article Background: The aim of the current study was to assess the mRNA levels of two mitochondria-related genes, including nuclear-encoded NRF1 (nuclear respiratory factor 1), mitochondrial transcription factor A (TFAM), and mitochondrial-encoded cytochrome c oxidase subunit 1 (MT-CO1) genes in various stages of the human oocyte maturation. Methods: Oocytes were obtained from nine infertile women with male factor undergoing in vitro fertilization (IVF)/intra-cytoplasmic sperm injection protocol. Mitochondrial-related mRNA levels were performed by single-cell TaqMan Real-time PCR. Results: the expression level of the target genes was low at the germinal vesicle stage (P>0.05). Although the mRNA level of NRF1 gene remained stable in metaphase I, the mRNA level of TFAM and MT-CO1 increased significantly (P<0.05).In metaphase II, the expression level of all genes increased compared to metaphase I (P<0.05). Conclusion: The overexpression levels of NRF1, TFAM, and MT-CO1 genes are related to the oocyte maturation. Therefore, the current study could be used clinically to improve the success rate of IVF. Transcription, Human oocyte, Real-time PCR 23 28 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-538&slc_lang=en&sid=1 Marefat Ghaffari Novin معرفت غفاری نوین No Mehrdad Noruzinia مهرداد نوروزی نیا No Azra Allahveisi عذرا الله ویسی allavaisie@gmail.com Yes Aboutaleb Saremi ابوطالب صارمی No Fateme Fadaei Fathabadi فاطمه فدایی فتح آبادی No Reza Mastery Farahani رضا ماستری فراهانی No Ali Dehghani Fard علی دهقانی فرد No Arash Pooladi آرش پولادی No Ramin Mazaherinezhad Fard رامین مظاهری نژاد فرد No Elham Yousefian الهام یوسفیان No