Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
-
10.61186/ibj
-
8888
-
en
jalali
1393
10
1
gregorian
2015
1
1
19
1
online
1
fulltext
en
مقایسه میزان رونویسی ژن های میتوکندریایی TFAM, NRF1 و MT-CO1 در مراحل مختلف بلوغ تخمک های انسان
Comparison of Mitochondrial-Related Transcriptional Levels of mitochondrial transcription factor A, Nuclear respiratory factor 1 and cytochrome c oxidase subunit 1 Genes in Single Human Oocytes at Various Stages of the Oocyte Maturation
Related Fields
Related Fields
مقاله کامل
Full Length/Original Article
Background: The aim of the current study was to assess the mRNA levels of two mitochondria-related genes, including nuclear-encoded NRF1 (nuclear respiratory factor 1), mitochondrial transcription factor A (TFAM), and mitochondrial-encoded cytochrome c oxidase subunit 1 (MT-CO1) genes in various stages of the human oocyte maturation. Methods: Oocytes were obtained from nine infertile women with male factor undergoing in vitro fertilization (IVF)/intra-cytoplasmic sperm injection protocol. Mitochondrial-related mRNA levels were performed by single-cell TaqMan Real-time PCR. Results: the expression level of the target genes was low at the germinal vesicle stage (P>0.05). Although the mRNA level of NRF1 gene remained stable in metaphase I, the mRNA level of TFAM and MT-CO1 increased significantly (P<0.05).In metaphase II, the expression level of all genes increased compared to metaphase I (P<0.05). Conclusion: The overexpression levels of NRF1, TFAM, and MT-CO1 genes are related to the oocyte maturation. Therefore, the current study could be used clinically to improve the success rate of IVF.
Transcription, Human oocyte, Real-time PCR
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http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-538&slc_lang=en&sid=1
Marefat
Ghaffari Novin
معرفت
غفاری نوین
100319475328460056047
100319475328460056047
No
Mehrdad
Noruzinia
مهرداد
نوروزی نیا
100319475328460056048
100319475328460056048
No
Azra
Allahveisi
عذرا
الله ویسی
allavaisie@gmail.com
100319475328460056049
100319475328460056049
Yes
Aboutaleb
Saremi
ابوطالب
صارمی
100319475328460056050
100319475328460056050
No
Fateme
Fadaei Fathabadi
فاطمه
فدایی فتح آبادی
100319475328460056051
100319475328460056051
No
Reza
Mastery Farahani
رضا
ماستری فراهانی
100319475328460056052
100319475328460056052
No
Ali
Dehghani Fard
علی
دهقانی فرد
100319475328460056053
100319475328460056053
No
Arash
Pooladi
آرش
پولادی
100319475328460056054
100319475328460056054
No
Ramin
Mazaherinezhad Fard
رامین
مظاهری نژاد فرد
100319475328460056055
100319475328460056055
No
Elham
Yousefian
الهام
یوسفیان
100319475328460056056
100319475328460056056
No