en بررسی HLA-G در لنفوسیت های B در بیماران لوسمی لنفوسیتیک مزمن Related Fields Related Fields مقاله کامل Full Length/Original Article The human leukocyte antigen G (HLA-G) molecule exhibits limited tissue distribution, low polymorphism and alternative splicings that generate seven HLA-G isoforms. HLA-G exerts multiple immunoregulatory functions. Recent studies indicate an ectopic up-regulation in tumor cells that may favor their escape from anti-tumor immune responses. This study it is an effort to clarify the presence of HLA-G in B-cell chronic lymphocytic leukemia (B-CLL) patients. Methods: HLA-G mRNA expression was studied in a pilot study in circulating B-CLL and also healthy controls by reverse transcription (RT)-PCR using a set of pan-HLA-gamma primers. Results: RT-PCR was performed on B-cells from 74 B-CLL patients and 12 healthy controls. The data showed HLA-G gene expression in 20% of the B-CLL patients. No expression of HLA-G could be detected in the healthy control group. Conclusion: These data suggest that HLA-G is expressed at the gene level in B cells from B-CLL patients but not in B cells from healthy controls. Further study is required to clarify the role of HLA-gamma as a regulatory factor that could affect immune response in B-CLL patients. Human leukocyte antigen G (HLA-G), Reverse transcription PCR (RT)-PCR, B-cell chronic lymphocytic leukemia (B-CLL) 125 129 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-89&slc_lang=en&sid=1 Mohammad Reza Rezvany محمدرضا رضوانی reza.rezvany@.ki.se Yes Azam Kazemi اعظم کاظمی No Abbas Hajifathali عباس حاجی فتحعلی No Saeed Kaviani سعید کاویانی No Hakan Mellstedt Håkan Mellstedt No