Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
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10.61186/ibj
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8888
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en
jalali
1385
10
1
gregorian
2007
1
1
11
1
online
1
fulltext
en
بیان افتراقی ایدیوتیپهای مشترک وابسته به فاکتور روماتوئید در بیماران ایرانی مبتلا به آرتریت روماتوئید سرم مثبت و سرم منفی.
Differential Expression of Rheumatoid Factor-Associated Cross-Reactive Idiotypes in Iranian Seropositive and Seronegative Patients with Rheumatoid Arthritis
Related Fields
Related Fields
مقاله کامل
Full Length/Original Article
High levels of rheumatoid factors (RF) are detectable in serum of the majority of patients with rheumatoid arthritis (RA), but 5-10% of patients remain seronegative (SN). Despite clinical and genetic similarities between these two subsets of RA, it has been proposed that they may be regarded as distinct clinical entities. Methods: In the present study a panel of monoclonal antibodies (mAb) recognizing RF-associated cross-reactive idiotypes (CRI) linked to the VH1 (G8), VH4 (LC1), VK3b (17-109) and a mAb recognizing the VK3 subgroup (C7) of immunoglobulin variable region (IgV) gene products were used to quantitate the level of expression of these gene products in serum and synovial fluid of 35 seropositive (SP) and 8 SN RA patients by capture ELISA. Results: While the concentration and relative proportion of the IgV are recognized by the mAb G8, 17-109 and C7 were significantly higher in serum and synovial fluid of the SP RA, compared to the SN-RA patients (G8, p = 0.009 17-109, p = 0.0001 C7, p = 0.001). The CRI recognized by the mAb LC1 was highly represented in serum and synovial fluid of the SN-RA patients. There have been no significant differences in the level of expression of these IgV gene products (other than the product recognized by C7 mAb in SP patients) between serum and synovial fluid of either group of patients. Conclusion: Our results suggest that the expressed repertoire of Ig VH and VK genes in these two subsets of RA is differentially regulated and may be influenced by selective mechanisms leading to positive or negative selection of certain genes.
Rheumatoid arthritis (RA), Rheumatoid factor (RF), Cross-reactive idiotypes (CRI), Seronegative (SN), Seropositive (SP)
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http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-81&slc_lang=en&sid=1
Ghasem
Mosayebi
قاسم
مسیبی
100319475328460057195
100319475328460057195
No
Mageed
Rizgar A.
مجید
ریزگر
100319475328460057196
100319475328460057196
No
Soheila
Gharagozloo
سهیلا
قره گوزلو
100319475328460057197
100319475328460057197
No
Fazel
Shokri
فاضل
شکری
100319475328460057198
100319475328460057198
Yes