Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
-
10.61186/ibj
-
8888
-
en
jalali
1393
1
1
gregorian
2014
4
1
18
2
online
1
fulltext
en
کریزین سمیت عصبی ناشی از آکریل آمید را در مطالعات برون تنی و درون تنی کاهش داد
Chrysin Reduced Acrylamide-Induced Neurotoxicity in Both in vitro and in vivo Assessments
Related Fields
Related Fields
مقاله کامل
Full Length/Original Article
Background: Acrylamide (ACR) is a well-known industrial toxic chemical that produces neurotoxicity, which is characterized by progressive central and peripheral neuronal degeneration. Chrysin is a natural, biologically active flavonoid compound, which is commonly found in many plants. The antioxidant and neuroprotective properties of chrysin have been demonstrated. Methods: In this study, the possible effect of chrysin on ACR-induced toxicity was evaluated in both in vitro and in vivo experiments. PC12 cells were used as a suitable in vitro model. Cells were exposed to chrysin (0.5-5 µM) for 12 and 24 h, and then ACR in IC50 concentration was added to the cells. Finally, cell viability was determined using (4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium assay. For in vivo assay, Wistar rats were treated with ACR (50 mg/kg i.p. for 11 days) alone or in combination with chrysin (12.5, 25, and 50 mg/kg). At the end of treatment, behavioral index was evaluated. Results: ACR decreased cell viability and pre-treatment with chrysin (0.5-5 µM) significantly decreased ACR-induced cytotoxicity in the time- and dose-dependent manner. In Wistar rats, exposure to ACR significantly induced severe gait abnormalities, but treatment with chrysin (50 mg/kg) reduced ACR-induced neurotoxicity in animals. Conclusion: In the current study, chrysin exhibited neuroprotective effect on PC12 cells as an in vitro model and also on Wistar rats.
Acrylamide, Chrysin, Neurotoxicity, Antioxidant
101
106
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-517&slc_lang=en&sid=1
Soghra
Mehri
صغری
مهری
100319475328460056017
100319475328460056017
No
Hamed
Veis Karami
حامد
ویس کرمی
100319475328460056018
100319475328460056018
No
Faezeh
Vahdati Hassani
فائزه
وحدتی حسنی
100319475328460056019
100319475328460056019
No
Hossein
Hosseinzadeh
حسین
حسین زاده
hosseinzadehh@mums.ac.ir
100319475328460056020
100319475328460056020
Yes