Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
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10.61186/ibj
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8888
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en
jalali
1386
10
1
gregorian
2008
1
1
12
1
online
1
fulltext
en
Induction of Apoptosis on K562 Cell Line and Double Strand Breaks on Colon Cancer Cell Line Expressing High Affinity Receptor for Granulocyte Macrophage-Colony Stimulating factor (GM-CSF)
Induction of Apoptosis on K562 Cell Line and Double Strand Breaks on Colon Cancer Cell Line Expressing High Affinity Receptor for Granulocyte Macrophage-Colony Stimulating factor (GM-CSF)
Related Fields
Related Fields
مقاله کامل
Full Length/Original Article
Background: Immunotoxins are comprised of both the cell targeting and the cell killing moieties. We previously established a new immunotoxin, i.e. Shiga toxin granulocyte macrophage-colony stimulating factor (StxA1-GM-CSF), comprises of catalytic domain of Stx, as a killing moiety and GM-CSF, as a cell targeting moiety. In this study, the ability of the immunotoxin to induce apoptosis and double strand breaks (DSB) on different cell lines was investigated. Methods: The recombinant hybrid protein was expressed in bacterial expression system and purified with nickel-nitrilotriacetate acid resin. The K562 (erythroid leukemia) cell line and LS174 (colon carcinoma) were used in this study. The neutral comet assay was carried out for the detection of DSB and Hoechst staining was performed for apoptosis. Results: StxA1-GM-CSF effectively induced apoptosis on K562 cell line and DNA Double Strand Break (DSB) were observed on colon cancer cell line treated with StxA1-GM-CSF. Conclusion: This novel action i.e. DNA damage might be a relevant mechanism of action for StxA1-GM-CSF that is designed to act as immunotoxin, although further investigation is required.
Shiga toxin granulocyte macrophage-colony stimulating factor (StxA1-GM-CSF), Cancer cells, Apoptosis, Double strand
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http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-65&slc_lang=en&sid=1
Mehryar
Habibi Roudkenar
مهریار
حبیبی رودکنار
100319475328460057057
100319475328460057057
No
Saeid
Bouzari
سعید
بوذری
saeidbouzari@yahoo.com
100319475328460057058
100319475328460057058
Yes
Yoshikazu
Kuwahara
Yoshikazu
Kuwahara
100319475328460057059
100319475328460057059
No
Amaneh
Mohammadi
آمنه
محمدی روشنده
100319475328460057060
100319475328460057060
No
Taisuke
Baba
Taisuke
Baba
100319475328460057061
100319475328460057061
No
Mana
Oloomi
مانا
علومی
100319475328460057062
100319475328460057062
No
Manabu
Fukumoto
Manabu
Fukumoto
100319475328460057063
100319475328460057063
No