Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
-
10.61186/ibj
-
8888
-
en
jalali
1392
7
1
gregorian
2013
10
1
17
4
online
1
fulltext
en
بیان و تخلیص پروتئین همراه نوروتوکسین (HA-33/A) کلستریدیوم بوتولینوم تیپ A و بررسی قابلیت آنتی ژنی آن
Expression and Purification of Neurotoxin-Associated Protein HA-33/A from Clostridium botulinum and Evaluation of Its Antigenicity
Related Fields
Related Fields
مقاله کامل
Full Length/Original Article
Background: Botulinum neurotoxin (BoNT) complexes consist of neurotoxin and neurotoxin-associated proteins. Hemagglutinin-33 (HA-33) is a member of BoNT type A (BoNT/A) complex. Considering the protective role of HA-33 in preservation of BoNT/A in gastrointestinal harsh conditions and also its adjuvant role, recombinant production of this protein is favorable. Thus in this study, HA-33 was expressed and purified, and subsequently its antigenicity in mice was studied. Methods: Initially, ha-33 gene sequence of Clostridium botulinum serotype A was adopted from GenBank. The gene sequence was optimized and synthesized in pET28a (+) vector. E. coli BL21 (DE3) strain was transformed by the recombinant vector and the expression of HA-33 was optimized at 37°C and 5 h induction time. Results: The recombinant protein was purified by nickel nitrilotriacetic acid agarose affinity chromatography and confirmed by immunoblotting. Enzyme Linked Immunoassay showed a high titer antibody production in mice. Conclusion: The results indicated a highly expressed and purified recombinant protein, which is able to evoke high antibody titers in mice.
Botulinum neurotoxin, Expression, Purification
165
170
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-489&slc_lang=en&sid=1
Ali
Sayadmanesh
علی
صیادمنش
100319475328460056236
100319475328460056236
No
Firouz
Ebrahimi
فیروز
ابراهیمی
febrhimi@ihu.ac.ir
100319475328460056237
100319475328460056237
Yes
Abbas
Hajizade
عباس
حاجی زاده
100319475328460056238
100319475328460056238
No
Mosayeb
Rostamian
مصیب
رستمیان
100319475328460056239
100319475328460056239
No
Hani
Keshavarz
هانی
کشاورز
100319475328460056240
100319475328460056240
No