TY - JOUR JF - انستیتو-پاستور-ایران JO - IBJ VL - 16 IS - 3 PY - 2012 Y1 - 2012/7/01 TI - Upregulation of Connexins 30 and 32 Gap Junctions in Rat Hippocampus at Transcription Level by Chronic Central Injection of Lipopolysaccharide TT - افزایش بیان ژن اتصالات شکافدار حاوی کانکسین های 30 و 32 در هیپوکمپ موشهای بزرگ آزمایشگاهی به دنبال تزریق مزمن و داخل مغزی لیپوپلی ساکارید N2 - Background: Gap junctions composed of connexins (Cx) are functional in cell defense by propagation of toxic/death molecules to neighboring cells. Hippocampus, one of the brain regions with particular vulnerability to damage, has a wide network of gap junctions. Functional response of astrocytic Cx30 and neuronal Cx32 to hippocampal damage is unknown. Methods: We infused lipopolysaccharide (LPS) intracerebroventricularly (2.5 µg/rat) once daily for two weeks to create neuroinflammation. The mRNA and protein levels of the Cx were measured in the hippocampus after 1st, 7th and 14th injection by real-time PCR and Western-blot techniques. Results: A significant increase in Cx32 and Cx30 gene expression was observed after 7th and 14th injection of LPS with no significant change in their protein abundance. Conclusion: Transcriptional overexpression of hippocampal Cx30 and Cx32 could be an adaptive response to production of intracellular toxic molecules but it is not accompanied with post- transcriptional overexpression and might have no functional impact. SP - 132 EP - 127 AU - Abbasian, Mohammad AU - Sayyah, Mohammad AU - Babapour, Vahab AU - Mahdian, Reza AU - Choopani, Samira AU - Kaviani, Bahar AD - KW - Connexin 30 KW - Connexin 32 KW - Hippocampus UR - http://ibj.pasteur.ac.ir/article-1-720-en.html DO - 10.6091/ibj.1099.2012 ER -