TY - JOUR JF - انستیتو-پاستور-ایران JO - IBJ VL - 25 IS - 5 PY - 2021 Y1 - 2021/9/01 TI - Mitochondrial DNA Copy Number Variations and Serum Pepsinogen Levels for Risk Assessment in Gastric Cancer TT - N2 - Background: Variations in mitochondrial DNA copy number (mtDNA-CN) of peripheral blood leukocytes (PBLs), as a potential biomarker for gastric cancer (GC) screening has currently been subject to controversy. Herein, we have assessed its efficiency in GC screening, in parallel and in combination with serum pepsinogen (sPG) I/II ratio, as an established indicator of gastric atrophy. Methods: The study population included GC (n = 53) and non-GC (n = 207) dyspeptic patients. The non-GC group was histologically categorized into CG (n = 104) and NM (n = 103) subgroups. The MtDNA-CN of PBLs was measured by quantitative real-time PCR. The sPG I and II levels and anti-H. pylori serum IgG were measured by ELISA. Results: The mtDNA-CN was found significantly higher in GC vs. non-GC (OR = 3.0; 95% CI = 1.4, 6.4) subjects. Conversely, GC patients had significantly lower sPG I/II ratio than the non-GC (OR = 3.2; CI = 1.4, 7.2) subjects. The combination of these two biomarkers yielded a dramatic amplification of the odds of GC risk in double-positive (high mtDNA-CN-low sPGI/II) subjects, in reference to double-negatives (low mtDNA-CN-high sPGI/II), when assessed against non-GC (OR = 27.1; CI = 5.0, 147.3), CG (OR = 13.1; CI = 2.4, 72.6), or NM (OR = 49.5; CI = 7.9, 311.6) groups. Conclusion: The combination of these two biomarkers, namely mtDNA-CN in PBLs and serum PG I/II ratio, drastically enhanced the efficiency of GC risk assessment, which calls for further validations. SP - 323 EP - 333 AU - Alikhani, Mehdi AU - Saberi, Samaneh AU - Esmaily, Maryam AU - Michel, Valérie AU - Tashakoripour, Mohammad AU - Abdirad, Afshin AU - Aghakhani, Arezoo AU - Eybpoosh, Sana AU - Vosough, Massoud AU - Mohagheghi, Mohammad Ali AU - Eshagh Hosseini, Mahmoud AU - Touati, Eliette AU - Mohammadi, Marjan AD - HPGC Research Group, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran KW - Biomarkers KW - DNA copy number variation KW - Mitochondrial DNA KW - Stomach neoplasmsr UR - http://ibj.pasteur.ac.ir/article-1-3395-en.html DO - 10.52547/ibj.25.5.323 ER -