TY - JOUR JF - انستیتو-پاستور-ایران JO - IBJ VL - 26 IS - 1 PY - 2022 Y1 - 2022/1/01 TI - Detection of Microsatellite Instability by High-Resolution Melting Analysis in Colorectal Cancer TT - N2 - Background: Colorectal cancer (CRC) is the third most common cancer worldwide. microsatellite instability (MSI) is a molecular marker of a deficient mismatch repair system and happens in almost 15% of CRCs. Because of a wide frequency of MSI+ CRC in Iran compared to other parts of the world, the importance of screening for this type of cancer is highlighted. Methods: The most common MSI detection technique is a fluorescent PCR-based method in which fragments are analyzed by capillary electrophoresis (CE). This technique is very time-consuming, difficult, and expensive. We sought to develop and evaluate a proper method with high accuracy, specificity, and sensitivity to screen the MSI+ CRC. A high-resolution melting (HRM) analysis procedure is relying on the analysis of the melting curve attributes. Low cost, feasibility, high specificity, and sensitivity are outstanding attributes of HRM analysis. Results: Five mononucleotide microsatellite markers, including BAT-25, BAT-26, NR-21, NR-24, and NR-27, in 25 archival CRC tumor tissue samples were compared with normal tissue adjacent using HRM method. The specificity and sensitivity of BAT-25 with HRM method were 100% compared to CE, while other markers had lower sensitivity. However, when all the markers were considered together, the sensitivity and specificity became 100%. The number of MSI+ samples was 56%, which shows a higher ratio than previous Iranian studies. The highest MSI was related to BAT-26 (52%). Conclusion: The HRM method is much simpler and more cost-effective than current MSI techniques, and its sensitivity and accuracy are comparable. Therefore, it can serve as an alternative method in cases where CE is unavailable. SP - 70 EP - 76 AU - Raji, Nafiseh AU - Majidi Zadeh, Tayebeh AU - Babaheidarian, Pegah AU - Houshmand, Massoud AD - National Institute of Genetic Engineering and Biotechnology KW - Biomarkers KW - Microsatellite instability KW - Colorectal cancer UR - http://ibj.pasteur.ac.ir/article-1-3062-en.html DO - 10.52547/ibj.26.1.70 ER -