@ARTICLE{Makvandi, author = {Mousavi-Nasab, Seyed Dawood and Sabahi, Farzaneh and Kaghazian, Hooman and Paryan, Mahdi and Mirab Samiee, Siamak and Ghaderi, Mostafa and Zali, Fatemeh and Makvandi, Manoochehr and }, title = {A Real-Time RT-PCR Assay for Genotyping of Rotavirus}, volume = {24}, number = {6}, abstract ={Background: HRV is the causative agent of severe gastroenteritis in children and responsible for two million hospitalizations and more than a half-million deaths annually. Sequence characteristics of the gene segments encoding the VP7 and VP4 proteins are used for the genotype classification of rotavirus. A wide variety of molecular methods are available, mainly based on reverse transcription PCR for rapid, specific and sensitive genotyping of rotaviruses. This study describes an alternative real-time PCR assay for genotyping of rotavirus. Methods: The samples of stools studied in this research have been collected from patients referred to Children's Medical Centers, Tehran, Iran. Rotavirus detection and genotyping were performed using the RT-PCR and semi-nested RT-PCR, respectively. Samples were then genotyped with a new real-time PCR. Results: The real-time PCR was able to genotype all positive samples with a mean Ct of 28.2. Besides, a concordance rate of 100% was detected between real-time PCR and semi-nested RT-PCR. Conclusion: In this study, the genotyping of rotavirus with real-time PCR showed that this method can provide several favorable features, including high sensitivity and specificity, and a wide dynamic range for rotavirus genotyping. }, URL = {http://ibj.pasteur.ac.ir/article-1-3158-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-3158-en.pdf}, journal = {Iranian Biomedical Journal}, doi = {10.29252/ibj.24.6.394}, year = {2020} }