TY - JOUR JF - انستیتو-پاستور-ایران JO - IBJ VL - 24 IS - 6 PY - 2020 Y1 - 2020/11/01 TI - The Predictive Value of Serum Cytokines for Distinguishing Celiac Disease from Non-Celiac Gluten Sensitivity and Healthy Subjects TT - N2 - Background: It has been established that the level of some inflammatory cytokines increases in celiac disease (CD) and non-celiac gluten sensitivity (NCGS) in comparison with healthy subjects. Therefore, the primary interest in our research was proposing an accurate tool to diagnose patients with CD and NCGS from healthy individuals in an Iranian population. Methods: The serum samples were examined in 171 participants, including 110 CD patients, 46 healthy individuals, and 15 NCGS. The commercial ELISA kits were used to detect the level of the following cytokines: IL-1, IL-6, IL-8, IL-15, and IFN-γ. The receiver operating characteristic (ROC) curve analysis was applied to determine the optimal thresholds for high sensitivity, specificity, positive and negative predictive values of cytokines, as the indicators of CD, NCGS, and healthy control groups. Results: In NCGS group, the values of area under the ROC curve for IL-1, IL-8, and IFN-γ were 71%, 78%, and 70%, respectively. To differentiate the CD and NCGS groups from the control group, IL-15 had the highest sensitivity (82.70%), specificity (56.50%), positive predictive value (81.98%), and negative predictive value (57.78%), followed by IL-8 with the highest sensitivity of 74.50%, specificity of 73.30%, and positive and negative predictive values of 95.35% and 30.21%, respectively. Conclusion: The obtained results demonstrate that IL-15 and IL-8 could be proposed as potential markers in their optimal cut-off points for distinguishing CD from the NCGS and the healthy control. Based on our findings, the evaluation of cytokine levels can be recommended as a useful tool for the diagnosis of CD and NCGS in a clinical practice. SP - 340 EP - 346 AU - Masaebi, Fatemeh AU - Azizmohammad Looha, Mehdi AU - Rostami-Nejad, Mohammad AU - Pourhoseingholi, Mohamad Amin AU - Mohseni, Navid AU - Samasca, Gabriel AU - Lupan, Iulia AU - Rezaei-Tavirani, Mostafa AU - Zali, Mohammad Reza AD - Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran KW - Celiac disease KW - Cytokines KW - Sensitivity and specificity UR - http://ibj.pasteur.ac.ir/article-1-3144-en.html DO - 10.29252/ibj.24.6.335 ER -