TY - JOUR JF - انستیتو-پاستور-ایران JO - IBJ VL - 22 IS - 5 PY - 2018 Y1 - 2018/9/01 TI - Detection of Somatic Mutation in Exon 12 of DNA Polymerase β in Ovarian Cancer Tissue Samples TT - N2 - Background: DNA polymerase β (pol β) is a key enzyme of base excision repair pathway. It is a 1-kb gene consisting of 14 exons. Its catalytic part lies between exon 8 and exon 14. Exon 12 has a role in deoxyribonucleotide triphosphate selection for nucleotide transferase activity. Methods: Genomic DNA was isolated from ovarian carcinoma samples. Single strand conformation polymorphism method was used to detect mutation in genomic DNA. Results: Twenty-four patients of the 152 pair of tumor samples (15.8%) exhibited a point mutation (C→G) in position 725 in exon 12, which shifts proline to arginine (P242R). Statistical analysis showed a significant (p < 0.001) relationship between pol β mutation and the age of detection. Conclusion: This is a newly reported somatic mutation of pol β in ovarian carcinoma patients from India. SP - 355 EP - 359 AU - Khanra, Kalyani AU - Choudhuri, Indranil AU - Bhattacharyya, Nandan AD - Department of Biotechnology, Panskura Banamali College, Panskura RS, PIN 721152, Purba Medinipur, West Bengal, India KW - DNA polymerase β KW - Single nucleotide polymorphism KW - Ovarian cancer UR - http://ibj.pasteur.ac.ir/article-1-2353-en.html DO - 10.29252/ibj.22.5.355 ER -