@ARTICLE{Darabi, author = {Shams Nooraei, Marzieh and Noori-Zadeh, Ali and Darabi, Shahram and Rajaei, Farzad and Golmohammadi, Zohreh and Abbaszadeh, Hojjat Allah and }, title = {Low Level of Autophagy-Related Gene 10 (ATG10) Expression in the 6-Hydroxydopamine Rat Model of Parkinson's Disease}, volume = {22}, number = {1}, abstract ={Background: Autophagy is a mechanism disassembling the damaged organelles from the cell. This study attempted to examine the expression of several autophagy-related genes in Parkinson’s disease (PD) rat model. Methods: The male Wistar rats were divided into three groups as control, sham, and lesion. In the latter group, the PD rat model was induced by the injection of 6-hydroxydopamine in the striatum. The behavioral test was conducted one (baseline) and four weeks after the surgery through apomorphine hydrochloride. Then the RT-PCR technique was employed to evaluate the expressions of p62/SQSTM, autophagy-related genes (ATG)5, ATG12, ATG16L1, ATG10, as well as GAPDH and LC3. Results: By injecting apomorphine, the striatal lesion group showed a significant contralateral rotation at fourth week as compared to the baseline. The examination of p62, ATG5, ATG12, ATG16L1, and LC3 expressions using RT-PCR revealed that p62, ATG5, ATG12, LC3, and ATG16L1 were expressed in the substantia nigra of PD rat model, while ATG10 was not expressed. Conclusion: ATG10 expression is necessary for the initiation of autophagy. Thus, these results show that autophagy deregulation occurs in the initiation stages of the process in the rat model of PD. }, URL = {http://ibj.pasteur.ac.ir/article-1-2128-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-2128-en.pdf}, journal = {Iranian Biomedical Journal}, doi = {10.22034/ibj.22.1.15}, year = {2018} }