%0 Journal Article %A Teimoori, Ali %A Nejati, Mehrab %A Ebrahimi, Saeedeh %A Makvandi, Manoochehr %A Zandi, Milad %A Azaran, Azarakhsh %T Analysis of NSP4 Gene and Its Association with Genotyping of Rotavirus Group A in Stool Samples %J Iranian Biomedical Journal %V 22 %N 1 %U http://ibj.pasteur.ac.ir/article-1-2115-en.html %R 10.22034/ibj.22.1.42 %D 2018 %K NSP4, Rotavirus, Genotyping, %X Background: Non-structural protein 4 (NSP4) is a critical protein for rotavirus (RV) replication and assembly. This protein has multiple domains and motifs that predispose its function and activity. NSP4 has a sequence divergence in human and animal RVs. Recently, 14 genotypes (E1-E14) of NSP4 have been identified, and E1 and E2 have been shown to be the most common genotypes in human. Methods: The gene and protein sequence of NSP4 in RV-positive samples were inspected with the aim of NSP4 genotyping and variation analysis in viroporin and other domains. P and G typings of RV samples were carried out by WHO primers using a semi-multiplex PCR method. Non-typeable RV samples were amplified by conserved primers and sequenced. Results: In viroporin and enterotoxin, conserved sequence was detected, and amino acids substitution with the same biochemical properties was found. Conclusion: Association of NSP4 genotype with P or G genotyping G1/G9 correlates with E1 genogroups. In electrophoretyping of RV, E2 genotype had a short pattern when compared to E1. %> http://ibj.pasteur.ac.ir/article-1-2115-en.pdf %P 42-49 %& 42 %! Gene Constellation of NSP4 and Genotyping of Rotavirus %9 Full Length/Original Article %L A-10-1-676 %+ Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran %G eng %@ 1028-852X %[ 2018