AU - Babaie, Jalal AU - Amiri, Samira AU - Homayoun, Robab AU - Azimi, Ebrahim AU - Mohabati, Reyhaneh AU - Berizi, Mahboubeh AU - Sadaie, M. Reza AU - Golkar, Majid TI - Immunization of C57BL/6 Mice with GRA2 Combined with MPL Conferred Partial Immune Protection against Toxoplasma gondii PT - JOURNAL ARTICLE TA - انستیتو-پاستور-ایران JN - انستیتو-پاستور-ایران VO - 22 VI - 1 IP - 1 4099 - http://ibj.pasteur.ac.ir/article-1-2095-en.html 4100 - http://ibj.pasteur.ac.ir/article-1-2095-en.pdf SO - انستیتو-پاستور-ایران 1 AB  - BBackground: We have previously reported that immunization with GRA 2 antigen of Toxoplasma gondii induces protective immunity in CBA /J (H2k) and BALB/c mice (H2d). We aimed to examine whether immunization of a distinct strain of rodent with recombinant dense granule antigens (GRA2) combined with monophosphorryl lipid A (MPL) adjuvant elicits protective immune response against T. gondii. Methods: C57BL/6 (H2b haplotype) mice were immunized with GRA 2, formulated in MPL adjuvant. Results: Strong humoral response, predominantly of IgG1 subclass and cellular response, IFN-γ, was detected at three weeks post immunization. Mice immunized with GRA 2 had significantly (p < 0.01) fewer brain cysts than those in the adjuvant group, upon challenge infection. Despite the production of a strong antibody response, IFN-γ production and brain cyst reduction were not significant when the immunized mice were infected four months after the immunization. Conclusions: We can conclude that GRA2 immunization partially protects against T. gondii infection in C57BL/6 mice, though the potency and longevity of this antigen as a standalone vaccine may vary in distinct genetic backgrounds. This observation further emphasizes the utility of GRA 2 for incorporation into a multi-antigenic vaccine against T. gondii. CP - IRAN IN - Molecular Parasitology Lab., Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran LG - eng PB - انستیتو-پاستور-ایران PG - 22 PT - Full Length/Original Article YR - 2018