TY - JOUR T1 - Main Quality Attributes of Monoclonal Antibodies and Effect of Cell Culture Components TT - Main Quality Attributes of Monoclonal Antibodies and Effect of Cell Culture Components JF - انستیتو-پاستور-ایران JO - انستیتو-پاستور-ایران VL - 21 IS - 3 UR - http://ibj.pasteur.ac.ir/article-1-1946-en.html Y1 - 2017 SP - 131 EP - 141 KW - Main quality attributes KW - Monoclonal antibodies KW - Glycosylation KW - Fragmentation N2 - The culture media optimization is an inevitable part of upstream process development in therapeutic monoclonal antibodies (mAbs) production. The quality by design (QbD) approach defines the assured quality of the final product through the development stage. An important step in QbD is determination of the main quality attributes. During the media optimization, some of the main quality attributes such as glycosylation pattern, charge variants, aggregates, and low-molecular-weight species, could be significantly altered. Here, we provide an overview of how cell culture medium components affects the main quality attributes of the mAbs. Knowing the relationship between the culture media components and the main quality attributes could be successfully utilized for a rational optimization of mammalian cell culture media for industrial mAbs production. M3 10.18869/acadpub.ibj.21.3.131 ER -