TY - JOUR T1 - The Effect of Starvation Stress on the Protein Profiles in Flexibacter chinensis TT - The Effect of Starvation Stress on the Protein Profiles in Flexibacter chinensis JF - انستیتو-پاستور-ایران JO - انستیتو-پاستور-ایران VL - 12 IS - 2 UR - http://ibj.pasteur.ac.ir/article-1-99-en.html Y1 - 2008 SP - 67 EP - 75 KW - Flexibacter chinensis KW - Starvation stress KW - Protein profile N2 - Background: Analysis of many proteins produced during the transition into the stationary phase and under stress conditions (including starvation stress) demonstrated that a number of novel proteins were induced in common to each stress and could be the reason for cross-protection in bacterial cells. It is necessary to investigate the synthesis of these proteins during different stress conditions. Methods: The changes in protein profile of Flexibacter chinensis at various stages of the starvation process and the other stresses were investigated using two-dimensional gel electrophoresis which has proven to be a powerful tool for investigation of the changes in protein profiles under such conditions. Results: Most starvation proteins were synthesized during the early stationary phase and many of these proteins remained during long-term starvation. Some of these proteins were transiently synthesized. The sequencing result of one of the proteins showed that there was a 62.5% identity in 8 amino acids overlapped with the 5’ residue of a 10 kDa chaperon protein which is known to be involved in the starvation stress response in other organisms. Conclusion: There are many proteins synthesized in common with many stresses in Flexibacter chinensis. Some of these proteins must play a major role in the stability of the cell under different stresses. M3 - ER -