%0 Journal Article %A Mehdipour Moghaddam, Mohammad Javad %A Mirbagheri, Adeleh Alsadat %A Salehi, Zivar %A Habibzade, Seyyed Mahmood %T Prevalence of Class 1 Integrons and Extended Spectrum Beta Lactamases among Multi-Drug Resistant Escherichia coli Isolates from North of Iran %J Iranian Biomedical Journal %V 19 %N 4 %U http://ibj.pasteur.ac.ir/article-1-1472-en.html %R 10.7508/ibj.2015.04.007 %D 2015 %K Antibiotic, Integrons, Escherichia coli, %X Background: Extended spectrum beta lactamases (ESBLs) are an important cause of transferable multidrug resistance (MDR) in gram-negative bacteria. The most described ESBL genes are generally found within integron-like structures as mobile genetic elements. The aim of this study was to identify the accompanying of class 1 integrons and ESBLs in the MDR E. coli isolates. Methods: Susceptibility to antimicrobial agents was determined for 33 E. coli strains by the disk diffusion method. Double-disk synergy test was applied for screening ESBL. To identify the strains carrying integrons, the conserved regions of integron-encoded integrase gene intI1 were amplified. For detection of gene cassettes, 5′CS and 3′CS primers were used. Results: All E. coli isolates were identified as multi-drug resistant. More than 50% of the isolates were resistant to tetracycline, cephalothin, cefuroxime, amoxicillin-clavulanic acid, and third generation cephalosporines. Nearly all of the isolates displayed sensitivity to piperacillin. There was a significant correlation between production of ESBL and resistance to all antibiotics except for ciprofloxacin and piperacillin (P < 0.01). Thirty two MDR strains (97%) included class 1 integron, and some isolates that included integrons were similar in the size of gene cassettes. The isolates were different in the resistance profiles however, some others had similar resistance profiles. Of eight ESBL positive isolates, seven (87.5%) carried class 1 integrons. Conclusion: Class 1 integrons were frequent in MDR and also ESBL-producing E. coli isolates. High prevalence of class 1 integrons confirms that integron-mediated antimicrobial gene cassettes are important in E. coli resistance profile. %> http://ibj.pasteur.ac.ir/article-1-1472-en.pdf %P 233-239 %& 233 %! Class 1 Integrons and ESBL among E. coli Isolates %9 Full Length/Original Article %L A-10-1-566 %+ %G eng %@ 1028-852X %[ 2015