%0 Journal Article %A Shams Mofarahe, Zahra %A Ghaffari Novin, Marefat %A Jafarabadi, Mina %A Salehnia, Mojdeh %A Noroozian, Mohsen %A Ghorbanmehr, Nassim %T Effect of Human Ovarian Tissue Vitrification/Warming on the Expression of Genes Related to Folliculogenesis %J Iranian Biomedical Journal %V 19 %N 4 %U http://ibj.pasteur.ac.ir/article-1-1451-en.html %R 10.7508/ibj.2015.04.005 %D 2015 %K Vitrification, Gene expression, Humans, %X Background: Ovarian tissue cryopreservation is an alternative strategy to preserve the fertility of women predicted to undergo premature ovarian failure. This study was designed to evaluate the expression of folliculogenesis-related genes, including factor in the germline alpha (FIGLA), growth differentiation factor-9 (GDF-9), follicle-stimulating hormone receptor (FSHR), and KIT LIGAND after vitrification/warming of human ovarian tissue. Methods: Human ovarian tissue samples were collected from five transsexual women. In the laboratory, the ovarian medullary part was removed by a surgical blade, and the cortical tissue was cut into small pieces. Some pieces were vitrified and warmed and the others were considered as non-vitrified group (control). Follicular normality was assessed with morphological observation by a light microscope, and the expression of FIGLA, KIT LIGAND, GDF-9,, and FSHR genes was examined using real-time RT-PCR in both the vitrified and non-vitrified groups. Results: Overall, 85% of the follicles preserved their normal morphologic feature after warming. The percentage of normal follicles and the expression of FIGLA, KIT LIGAND, GDF-9, and FSHR genes were similar in both vitrified and non-vitrified groups (P > 0.05). Conclusion: Vitrification/warming of human ovarian tissue had no remarkable effect on the expression of folliculogenesis-related genes. %> http://ibj.pasteur.ac.ir/article-1-1451-en.pdf %P 220-225 %& 220 %! Follicular Gene Expression after Ovarian Tissue Vitrification %9 Full Length/Original Article %L A-10-1-563 %+ %G eng %@ 1028-852X %[ 2015