TY - JOUR JF - انستیتو-پاستور-ایران JO - IBJ VL - 18 IS - 1 PY - 2014 Y1 - 2014/1/01 TI - The Effects of Fibroblast Co-Culture and Activin A on in vitro Growth of Mouse Preantral Follicles TT - تاثیر همکشتی فیبروبلاست و Activin A بر رشد آزمایشگاهی فولیکول های پره آنترال موش N2 - Background: This study was conducted to evaluate fibroblast co-culture and Activin A on in vitro maturation and fertilization of mouse preantral follicles. Methods: The ovaries from 12-14-day-old mice were dissected, and 120-150 μm preantral follicles were cultured individually in α-MEM as based medium for 12 days. A total number of 456 follicles were cultured in four conditions: (i) base medium as control group (n = 113), (ii) base medium supplemented with 30 ng/ml Activin A (n = 115), (iii) base medium co-cultured with mouse embryonic fibroblast (n = 113), and (iv) base medium supplemented with 30 ng/ml Activin A and co-cultured with fibroblast (n = 115). Rate of growth, survivability, antrum formation, ovulation, embryonic development and steroid production were evaluated. Analysis of Variance and Duncan test were applied for analyzing. Results: Both co-culture and co-culture + Activin A groups showed significant difference (P<0.05) in growth (on days 4, 6, and 8 of culture period) and survival rates. However, there was no significant difference in antrum formation, ovulation rate, and embryonic development of ovulated oocytes. There were significant differences (P<0.05) in the estradiol production on days 8, 10, and 12 between co-culture + Activin A and the control group. Progesterone production also was significant (P<0.05) in co-culture + Activin A group on days 6, 8, 10, and 12 compared to control group. Conclusion: Fibroblast co-culture and Activin A promoted growth and survivability of preantral follicles. However, simultaneous use of them was more efficient. SP - 49 EP - 54 AU - Karimpour Malekshah, Abbasali AU - Heidari, Mahmoud AU - Parivar, Kazem AU - Azami, Nasrin Sadat AD - KW - Fibroblast KW - Activin A KW - Follicle UR - http://ibj.pasteur.ac.ir/article-1-1129-en.html DO - 10.6091/ibj.12642.2013 ER -