TY - JOUR T1 - The Effect of Human Platelet-Rich Plasma on Adipose-Derived Stem Cell Proliferation and Osteogenic Differentiation TT - اثرات پلاسمای غنی از پلاکت بر تزاید و تمایز به استئوبلاست در سلولهای بنیادی مشتق از بافت چربی انسانی JF - انستیتو-پاستور-ایران JO - انستیتو-پاستور-ایران VL - 18 IS - 3 UR - http://ibj.pasteur.ac.ir/article-1-1114-en.html Y1 - 2014 SP - 151 EP - 157 KW - Platelet-Rich Plasma KW - Adipose tissue KW - Stem Cells KW - Cell differentiation KW - Cell proliferation N2 - Background: The cultured mesenchymal stem cells (MSC) have been used in many clinical trials however, there are still some concerns about the cultural conditions. One concern is related to the use of FBS as a widely used xenogeneic supplement in the culture system. Human platelet-rich plasma (hPRP) is a candidate replacement for FBS. In this study, the effect of hPRP on MSC proliferation and osteogenic differentiation has been evaluated. Methods: Human adipose-derived stem cells (hADSC) were expanded. Cells from the third passage were characterized by flow cytometric analysis and used for in vitro experiments. Resazurin and alizarin red stains were used for cell proliferation and osteogenic differentiation assays, respectively. Results: Treatment with hPRP resulted in a statistically significant increase in cell proliferation compare to the negative control group (P<0.001). Cell proliferation in the 15% hPRP group was also significantly higher than that in the 10% hPRP group (P<0.05). Additionally, it caused less osteogenic differentiation of the hADSC compared to the FBS (P<0.001), but in comparison to negative control, it caused acceptable mineralization (P<0.001). Conclusion: These findings indicate that hPRP not only improves the proliferation but also it can be a suitable substitution in osteogenic differentiation for clinical purposes. However, the clinical application value of hPRP still needs more investigation. M3 10.6091/ibj.13012.2014 ER -