TY - JOUR T1 - Human Ovarian Tissue Vitrification/Warming Has Minor Effect on the Expression of Apoptosis-Related Genes TT - انجماد شیشه ای و ذوب بافت تخمدان انسانی تاثیر اندکی بر بیان ژنهای وابسته به آپوپتوز دارد JF - انستیتو-پاستور-ایران JO - انستیتو-پاستور-ایران VL - 17 IS - 4 UR - http://ibj.pasteur.ac.ir/article-1-1011-en.html Y1 - 2013 SP - 179 EP - 186 KW - Vitrification KW - Apoptosis KW - Gene expression KW - Ovary KW - Humans N2 - Background: In this study, we evaluated the incidence of apoptosis at the ultrastructural levels and expression of some apoptosis-related genes in vitrified human ovarian tissue just after warming. Methods: Human ovarian tissue biopsies from 23 women after caesarean section were transported to the laboratory within 2 hours, and then they were cut into small pieces. Some pieces were vitrified and warmed and the other samples were considered as control. Apoptosis was assessed by a transmission electron microscope and also by molecular analysis of pro-apoptotic (Fas, FasL, Bax, p53, caspase8, and caspase3) and antiapoptotic (Bcl-2 and BIRC5) genem RNA levels using real-time RT-PCR before and after vitrification. Results: No sign of apoptosis was shown ultrastructurally in vitrified samples. The level of FasL, Bcl-2, Bax, p53, and caspase3 mRNA and Bax:Bcl-2 ratio were similar in non-vitrified and vitrified groups however, the expression of Fas and caspase8 genes was higher and BIRC5 was lower in vitrified samples compared to non-vitrified group (P<0.05). Conclusion: The fine structure of human vitrified ovarian tissue was well preserved moreover, vitrification was shown to affect the expression of some apoptosis-related genes. However, additional study is needed to confirm this observation. M3 10.6091/ibj.1243.2013 ER -