RT - Journal Article T1 - Cloning and Expression of Thermus Aquaticus DNA Polymerase Gene, Using a Thermo-Inducible Expression Vector JF - انستیتو-پاستور-ایران YR - 1998 JO - انستیتو-پاستور-ایران VO - 2 IS - 2 UR - http://ibj.pasteur.ac.ir/article-1-870-en.html SP - 79 EP - 82 K1 - Taq DNA polymerase K1 - PCR K1 - Thermal induction AB - DNA polymerase gene from Thermus aquaticus strain YT1 was amplified using VENTTM DNA po-lymerase and cloned under the control of X.PR promoter and expression was induced by a shift in tern perature. The culture was then sonicated, and after centrifugation the lysate was treated with poly‌ethyleneimine followed by a salting-out step. Finally the protein was precipitated with ammonium sulfate and fractionated by gel filtration. The resulting enzyme preparation was stable and active. LA eng UL http://ibj.pasteur.ac.ir/article-1-870-en.html M3 ER -