AU - Bouzari, Saeid AU - Rechinsky, Veladimir O. TI - Cloning and Expression of Thermus Aquaticus DNA Polymerase Gene, Using a Thermo-Inducible Expression Vector PT - JOURNAL ARTICLE TA - انستیتو-پاستور-ایران JN - انستیتو-پاستور-ایران VO - 2 VI - 2 IP - 2 4099 - http://ibj.pasteur.ac.ir/article-1-870-en.html 4100 - http://ibj.pasteur.ac.ir/article-1-870-en.pdf SO - انستیتو-پاستور-ایران 2 AB  - DNA polymerase gene from Thermus aquaticus strain YT1 was amplified using VENTTM DNA po-lymerase and cloned under the control of X.PR promoter and expression was induced by a shift in tern perature. The culture was then sonicated, and after centrifugation the lysate was treated with poly‌ethyleneimine followed by a salting-out step. Finally the protein was precipitated with ammonium sulfate and fractionated by gel filtration. The resulting enzyme preparation was stable and active. CP - IRAN IN - LG - eng PB - انستیتو-پاستور-ایران PG - 79 PT - Full Length/Original Article YR - 1998