%0 Journal Article %A Bouzari, Saeid %A Rechinsky, Veladimir O. %T Cloning and Expression of Thermus Aquaticus DNA Polymerase Gene, Using a Thermo-Inducible Expression Vector %J Iranian Biomedical Journal %V 2 %N 2 %U http://ibj.pasteur.ac.ir/article-1-870-en.html %R %D 1998 %K Taq DNA polymerase, PCR, Thermal induction, %X DNA polymerase gene from Thermus aquaticus strain YT1 was amplified using VENTTM DNA po-lymerase and cloned under the control of X.PR promoter and expression was induced by a shift in tern perature. The culture was then sonicated, and after centrifugation the lysate was treated with poly‌ethyleneimine followed by a salting-out step. Finally the protein was precipitated with ammonium sulfate and fractionated by gel filtration. The resulting enzyme preparation was stable and active. %> http://ibj.pasteur.ac.ir/article-1-870-en.pdf %P 79-82 %& 79 %! %9 Full Length/Original Article %L A-10-1-438 %+ %G eng %@ 1028-852X %[ 1998